Document Detail


Mitochondrial loss indicates early axonal damage in small fiber neuropathies.
MedLine Citation:
PMID:  22734900     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Evaluation of nerve fibers in the skin provides a useful tool for the diagnosis of small fiber neuropathies (SFNs). Our aim was to determine whether mitochondria are involved in SFN, indicating early axonal damage. We quantified mitochondrial respiratory chain complex IV (OXPHOS) and axonal (PGP 9.5) fluorescence on skin sections from 32 SFN patients and 14 healthy controls. Also, a group of six patients were recruited before and after 30-day treatment with the mitotoxic antibiotic linezolid. We measured the co-localization of OXPHOS within the intraepidermal and subpapillary dermal axons (PGP-immunoreactive [PGP-ir]). SFN patients with relatively preserved intraepidermal nerve fibers (SFN borderline) showed statistically significant reduction of OXPHOS (50.5 ± 33.9 µm(2) vs. 107.6 ± 81 µm(2) in controls, p < 0.02). A positive correlation was found between both PGP-ir and OXPHOS in controls (Pearson's coefficient r = 0.59, p < 0.001), whereas such correlation was absent in SFN. With respect to baseline measurements, linezolid therapy increased both PGP-ir and OXPHOS, which could be considered an initial compensatory toxic-induced response. This study set out to identify a possible marker of axonal pre-degenerative state in SFN borderline patients.
Authors:
Jordi Casanova-Molla; Merche Morales; Glòria Garrabou; Núria Solà-Valls; Alex Soriano; Maria Calvo; Josep Maria Grau; Josep Valls-Solé
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Journal of the peripheral nervous system : JPNS     Volume:  17     ISSN:  1529-8027     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9704532     Medline TA:  J Peripher Nerv Syst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  147-157     Citation Subset:  -    
Copyright Information:
© 2012 Peripheral Nerve Society.
Affiliation:
Department of Neurology, Hospital Clinic IDIBAPS-Institut d'Investigació Augustí Pi i Sunyer, University of Barcelona Mitochondrial Functionalism Unit, Department of Internal Medicine, Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Hospital Clinic Department of Infectious Diseases, Hospital Clinic Confocal Microscopy Unit, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
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