Document Detail

Mitochondrial localization of the low level p53 protein in proliferative cells.
MedLine Citation:
PMID:  19635458     Owner:  NLM     Status:  MEDLINE    
p53 protein plays a central role in suppressing tumorigenesis by inducing cell cycle arrest or apoptosis through transcription-dependent and -independent mechanisms. Emerging publications suggest that following stress, a fraction of p53 translocates to mitochondria to induce cytochrome c release and apoptosis. However, the localization of p53 under unstressed conditions remains largely unexplored. Here we show that p53 is localized at mitochondria in absence of apoptotic stimuli, when cells are proliferating, localization observed in various cell types (rodent and human). This is also supported by acellular assays in which p53 bind strongly to mitochondria isolated from rat liver. Furthermore, the mitochondria subfractionation study and the alkaline treatment of the mitochondrial p53 revealed that the majority of mitochondrial p53 is present in the membranous compartments. Finally, we identified VDAC, a protein of the mitochondrial outer-membrane, as a putative partner of p53 in unstressed/proliferative cells.
Ioana Ferecatu; Marie Bergeaud; Aida Rodríguez-Enfedaque; Nathalie Le Floch; Lisa Oliver; Vincent Rincheval; Flore Renaud; François M Vallette; Bernard Mignotte; Jean-Luc Vayssière
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-25
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  387     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-08-18     Completed Date:  2009-08-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  772-7     Citation Subset:  IM    
CNRS UMR, Université de Versailles Saint-Quentin-en-Yvelines, France.
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MeSH Terms
Cell Line
Cell Proliferation*
Mitochondria / metabolism*
Tumor Suppressor Protein p53 / metabolism*
Reg. No./Substance:
0/Tumor Suppressor Protein p53

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