| Mitochondrial glycerol-3-phosphate acyltransferase-deficient mice have reduced weight and liver triacylglycerol content and altered glycerolipid fatty acid composition. | |
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MedLine Citation:
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PMID: 12417724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microsomal and mitochondrial isoforms of glycerol-3-phosphate acyltransferase (GPAT; E.C. 2.3.1.15) catalyze the committed step in glycerolipid synthesis. The mitochondrial isoform, mtGPAT, was believed to control the positioning of saturated fatty acids at the sn-1 position of phospholipids, and nutritional, hormonal, and overexpression studies suggested that mtGPAT activity is important for the synthesis of triacylglycerol. To determine whether these purported functions were true, we constructed mice deficient in mtGPAT. mtGPAT(-/-) mice weighed less than controls and had reduced gonadal fat pad weights and lower hepatic triacylglycerol content, plasma triacylglycerol, and very low density lipoprotein triacylglycerol secretion. As predicted, in mtGPAT(-/-) liver, the palmitate content was lower in triacylglycerol, phosphatidylcholine, and phosphatidylethanolamine. Positional analysis revealed that mtGPAT(-/-) liver phosphatidylethanolamine and phosphatidylcholine had about 21% less palmitate in the sn-1 position and 36 and 40%, respectively, more arachidonate in the sn-2 position. These data confirm the important role of mtGPAT in the synthesis of triacylglycerol, in the fatty acid content of triacylglycerol and cholesterol esters, and in the positioning of specific fatty acids, particularly palmitate and arachidonate, in phospholipids. The increase in arachidonate may be functionally significant in terms of eicosanoid production. |
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Authors:
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Linda E Hammond; Patricia A Gallagher; Shuli Wang; Sylvia Hiller; Kimberly D Kluckman; Eugenia L Posey-Marcos; Nobuyo Maeda; Rosalind A Coleman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 22 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-11-05 Completed Date: 2003-01-02 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: 8204-14 Citation Subset: IM |
Affiliation:
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Department of Nutrition. Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA. rcoleman@unc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Body Weight* Female Gene Targeting Glycerol-3-Phosphate O-Acyltransferase / genetics*, metabolism* Glycerophospholipids / chemistry, metabolism* Isoenzymes / genetics, metabolism Liver / metabolism* Male Mice Mice, Knockout Mitochondria / enzymology* Triglycerides / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK56350/DK/NIDDK NIH HHS; DK56598/DK/NIDDK NIH HHS; GM20920/GM/NIGMS NIH HHS; HL42630/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycerophospholipids; 0/Isoenzymes; 0/Triglycerides; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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