Document Detail


Mitochondrial glycerol-3-phosphate acyltransferase-deficient mice have reduced weight and liver triacylglycerol content and altered glycerolipid fatty acid composition.
MedLine Citation:
PMID:  12417724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microsomal and mitochondrial isoforms of glycerol-3-phosphate acyltransferase (GPAT; E.C. 2.3.1.15) catalyze the committed step in glycerolipid synthesis. The mitochondrial isoform, mtGPAT, was believed to control the positioning of saturated fatty acids at the sn-1 position of phospholipids, and nutritional, hormonal, and overexpression studies suggested that mtGPAT activity is important for the synthesis of triacylglycerol. To determine whether these purported functions were true, we constructed mice deficient in mtGPAT. mtGPAT(-/-) mice weighed less than controls and had reduced gonadal fat pad weights and lower hepatic triacylglycerol content, plasma triacylglycerol, and very low density lipoprotein triacylglycerol secretion. As predicted, in mtGPAT(-/-) liver, the palmitate content was lower in triacylglycerol, phosphatidylcholine, and phosphatidylethanolamine. Positional analysis revealed that mtGPAT(-/-) liver phosphatidylethanolamine and phosphatidylcholine had about 21% less palmitate in the sn-1 position and 36 and 40%, respectively, more arachidonate in the sn-2 position. These data confirm the important role of mtGPAT in the synthesis of triacylglycerol, in the fatty acid content of triacylglycerol and cholesterol esters, and in the positioning of specific fatty acids, particularly palmitate and arachidonate, in phospholipids. The increase in arachidonate may be functionally significant in terms of eicosanoid production.
Authors:
Linda E Hammond; Patricia A Gallagher; Shuli Wang; Sylvia Hiller; Kimberly D Kluckman; Eugenia L Posey-Marcos; Nobuyo Maeda; Rosalind A Coleman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  22     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-05     Completed Date:  2003-01-02     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8204-14     Citation Subset:  IM    
Affiliation:
Department of Nutrition. Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA. rcoleman@unc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Body Weight*
Female
Gene Targeting
Glycerol-3-Phosphate O-Acyltransferase / genetics*,  metabolism*
Glycerophospholipids / chemistry,  metabolism*
Isoenzymes / genetics,  metabolism
Liver / metabolism*
Male
Mice
Mice, Knockout
Mitochondria / enzymology*
Triglycerides / metabolism*
Grant Support
ID/Acronym/Agency:
DK56350/DK/NIDDK NIH HHS; DK56598/DK/NIDDK NIH HHS; GM20920/GM/NIGMS NIH HHS; HL42630/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Glycerophospholipids; 0/Isoenzymes; 0/Triglycerides; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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