Document Detail

Mitochondrial function after asphyxia in newborn lambs.
MedLine Citation:
PMID:  2718209     Owner:  NLM     Status:  MEDLINE    
We examined mitochondrial oxidative function 5 minutes and 2 hours after a gradual asphyxial insult in newborn lambs. We subjected 16 ventilated newborn lambs to 75-90 minutes of hypoxia and hypercarbia that resulted in bradycardia and systemic hypotension over the final 15 minutes of the insult. At the end of asphyxia, the lambs were resuscitated and returned to control ventilator settings. Samples of brain were removed 5 minutes (n = 8) and 2 hours (n = 8) after asphyxia. Each group of eight lambs was subdivided into those less than 3 or greater than 3 days old to evaluate the effect of age on postasphyxia mitochondrial function. After classification into nonsynaptic and synaptic mitochondria, mitochondrial respiration (oxygen consumption) was measured using five different substrates. Data from asphyxiated lambs were compared with that from a control group of ventilated nonasphyxiated lambs (n = 8). In the lambs less than 3 days old, there was significant depression of mean +/- SEM nonsynaptic mitochondrial state 3 (adenosine diphosphate-dependent) respiration to 29.5 +/- 5.2% of control with four of the five substrates and of state 4 respiration to 33.7 +/- 0.9% of control with three of the five substrates 5 minutes after asphyxia. By 2 hours after asphyxia, mean +/- SEM nonsynaptic mitochondria state 3 respiration increased to 70.4 +/- 6.4% of control while state 4 respiration increased to 58.2 +/- 4.5% of control. In contrast, lambs greater than 3 days old exhibited no inhibition of nonsynaptic mitochondrial function after asphyxia.(ABSTRACT TRUNCATED AT 250 WORDS)
A A Rosenberg; J K Parks; E Murdaugh; W D Parker
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  20     ISSN:  0039-2499     ISO Abbreviation:  Stroke     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-06-12     Completed Date:  1989-06-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  674-9     Citation Subset:  IM    
Division of Perinatal Medicine, University of Colorado School of Medicine, Denver 80262.
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MeSH Terms
Adenosine Diphosphate / metabolism
Animals, Newborn
Asphyxia Neonatorum / blood*,  physiopathology
Blood Pressure
Brain / metabolism*
Infant, Newborn
Mitochondria / metabolism*
Synapses / metabolism
Grant Support
Reg. No./Substance:
58-64-0/Adenosine Diphosphate

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