| Mitochondrial fatty acid synthesis is required for normal mitochondrial morphology and function in Trypanosoma brucei. | |
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MedLine Citation:
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PMID: 18221265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trypanosoma brucei use microsomal elongases for de novo synthesis of most of its fatty acids. In addition, this parasite utilizes an essential mitochondrial type II synthase for production of octanoate (a lipoic acid precursor) as well as longer fatty acids such as palmitate. Evidence from other organisms suggests that mitochondrially synthesized fatty acids are required for efficient respiration but the exact relationship remains unclear. In procyclic form trypanosomes, we also found that RNAi depletion of the mitochondrial acyl carrier protein, an important component of the fatty acid synthesis machinery, significantly reduces cytochrome-mediated respiration. This reduction was explained by RNAi-mediated inhibition of respiratory complexes II, III and IV, but not complex I. Other effects of RNAi, such as changes in mitochondrial morphology and alterations in membrane potential, raised the possibility of a change in mitochondrial membrane composition. Using mass spectrometry, we observed a decrease in total and mitochondrial phosphatidylinositol and mitochondrial phosphatidylethanolamine. Thus, we conclude that the mitochondrial synthase produces fatty acids needed for maintaining local phospholipid levels that are required for activity of respiratory complexes and preservation of mitochondrial morphology and function. |
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Authors:
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Jennifer L Guler; Eva Kriegova; Terry K Smith; Julius Lukes; Paul T Englund |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-01-23 |
Journal Detail:
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Title: Molecular microbiology Volume: 67 ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-14 Completed Date: 2008-05-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1125-42 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyl Carrier Protein
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antagonists & inhibitors,
genetics,
metabolism* Animals Chromatography, Thin Layer Electron Transport Complex II / genetics, metabolism Electron Transport Complex III / genetics, metabolism Electron Transport Complex IV / genetics, metabolism Fatty Acids / analysis, metabolism* Mass Spectrometry Membrane Potential, Mitochondrial Microscopy, Electron Mitochondria / chemistry, metabolism*, ultrastructure* Mitochondrial Proteins / genetics, metabolism Oxygen / metabolism Phospholipids / analysis, metabolism Protozoan Proteins / genetics, metabolism RNA Interference Trypanosoma brucei brucei / growth & development, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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067441//Wellcome Trust; AI21334/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acyl Carrier Protein; 0/Fatty Acids; 0/Mitochondrial Proteins; 0/Phospholipids; 0/Protozoan Proteins; 7782-44-7/Oxygen; EC 1.10.2.2/Electron Transport Complex III; EC 1.3.5.1/Electron Transport Complex II; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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