Document Detail


Mitochondrial dysfunction in congenital nephrotic syndrome.
MedLine Citation:
PMID:  10950113     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The molecular mechanisms maintaining the kidney glomerular filtration barrier remain poorly understood. Recent evidence suggests that mitochondrial dysfunction is a characteristic feature of kidney glomeruli in congenital nephrotic syndrome of the Finnish type (CNF). Here we searched for detailed functional evidence of mitochondrial lesion in CNF kidneys. We used histochemical and immunohistochemical methods, quantitative measurement of mitochondrial DNA, and superoxide production to characterize the mitochondrial function. The results unequivocally show down-regulation of mitochondria-encoded respiratory chain components, whereas the respective nuclearly encoded subunits were close to normal. These results give detailed evidence of distinct mitochondrial dysfunction and of the resulting abnormal production of reactive oxygen species in CNF and suggest a critical role for mitochondria in maintaining the glomerular permeability barrier.
Authors:
M L Solin; S Pitkänen; J W Taanman; H Holthöfer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  80     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-08-31     Completed Date:  2000-08-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1227-32     Citation Subset:  IM    
Affiliation:
Haartman Institute, Division of Bacteriology and Immunology, University of Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
DNA, Mitochondrial / genetics
Electron Transport
Electron Transport Complex IV / metabolism
Humans
Mitochondria / metabolism,  physiology*
NADH Dehydrogenase / metabolism
Nephrotic Syndrome / congenital*,  metabolism,  physiopathology*
Reactive Oxygen Species
Succinate Cytochrome c Oxidoreductase / metabolism
Superoxides / metabolism
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; EC 1.-/Succinate Cytochrome c Oxidoreductase; EC 1.6.99.3/NADH Dehydrogenase; EC 1.9.3.1/Electron Transport Complex IV

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