Document Detail

Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging.
MedLine Citation:
PMID:  18522988     Owner:  NLM     Status:  MEDLINE    
Visualization of organelles in living cells is a powerful method for studying their dynamic behavior. Here we attempted to visualize mitochondria in angiosperm male gametophyte (pollen grain from Arabidopsis thaliana) that are composed of one vegetative cell (VC) and two sperm cells (SCs). Combination of mitochondria-targeted fluorescent proteins with VC- or SC-specific expression allowed us to observe the precise number and dynamic behavior of mitochondria in the respective cell types. Furthermore, live imaging of SC mitochondria during double fertilization confirmed previous observations, demonstrated by electron microscopy in other species, that sperm mitochondria enter into the egg and central cells. We also attempted to visualize mutant mitochondria that were elongated due to a defect in mitochondrial division. This mutant phenotype was indeed detectable in VC mitochondria of a heterozygous F(1) plant, suggesting active mitochondrial division in male gametophyte. Finally, we performed mutant screening and isolated a putative mitochondrial protein transport mutant whose phenotype was detectable only in haploid cells. The transgenic materials presented in this work are useful not only for live imaging but also for studying mitochondrial functions by mutant analysis.
Ryo Matsushima; Yuki Hamamura; Tetsuya Higashiyama; Shin-ichi Arimura; Sodmergen; Nobuhiro Tsutsumi; Wataru Sakamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-03
Journal Detail:
Title:  Plant & cell physiology     Volume:  49     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1074-83     Citation Subset:  IM    
Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, 710-0046 Japan.
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MeSH Terms
Arabidopsis / cytology*,  genetics
Arabidopsis Proteins / metabolism
Crosses, Genetic
Germ Cells / cytology*
Green Fluorescent Proteins / metabolism
Microscopy, Fluorescence
Mitochondria / metabolism*
Mutant Proteins / isolation & purification,  metabolism
Mutation / genetics
Plants, Genetically Modified
Pollen / cytology*
Protein Transport
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Mutant Proteins; 0/dynamin-related protein DRP3A, Arabidopsis; 147336-22-9/Green Fluorescent Proteins

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