| Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: implications for developmental potential. | |
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MedLine Citation:
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PMID: 16945363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondrial distribution and microtubule organization were examined in porcine oocytes after parthenogenesis, fertilization and somatic cell nuclear transfer (SCNT). Our results revealed that mitochondria are translocated from the oocyte's cortex to the perinuclear area by microtubules that either constitute the sperm aster in in vitro-fertilized (IVF) oocytes or originate from the donor cell centrosomes in SCNT oocytes. The ability to translocate mitochondria to the perinuclear area was lower in SCNT oocytes than in IVF oocytes. Sperm-induced activation rather than electrical activation of SCNT oocytes as well as the presence of the oocyte spindle enhanced perinuclear mitochondrial association with reconstructed nuclei, while removal of the oocyte spindle prior to sperm penetration decreased mitochondrial association with male pronuclei without having an apparent effect on microtubules. We conclude that factors derived from spermatozoa and oocyte spindles may affect the ability of zygotic microtubules to translocate mitochondria after IVF and SCNT in porcine oocytes. Mitochondrial association with pronuclei was positively related with embryo development after IVF. The reduced mitochondrial association with nuclei in SCNT oocytes may be one of the reasons for the low cloning efficiency which could be corrected by adding yet to be identified, sperm-derived factors that are normally present during physiological fertilization. |
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Authors:
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Mika Katayama; Zhisheng Zhong; Liangxue Lai; Peter Sutovsky; Randall S Prather; Heide Schatten |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-07-28 |
Journal Detail:
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Title: Developmental biology Volume: 299 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-18 Completed Date: 2006-11-29 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 206-20 Citation Subset: IM |
Affiliation:
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Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri-Columbia, MO, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Nucleus / drug effects, metabolism Electric Stimulation Embryo, Mammalian / cytology, drug effects, metabolism* Female Fertilization in Vitro / methods* Fetus / cytology, drug effects Fibroblasts / cytology, drug effects Hydrogen-Ion Concentration Male Microtubules / drug effects, metabolism* Mitochondria / drug effects, metabolism* Mitotic Spindle Apparatus / drug effects, metabolism Nocodazole / pharmacology Nuclear Transfer Techniques Oocytes / cytology, drug effects Research Embryo Creation / methods* Sperm-Ovum Interactions Swine |
| Grant Support | |
ID/Acronym/Agency:
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R01-RR13438-07/RR/NCRR NIH HHS; R03 HD043829-02/HD/NICHD NIH HHS; R03-HD43829-02/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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31430-18-9/Nocodazole |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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