Document Detail


Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: implications for developmental potential.
MedLine Citation:
PMID:  16945363     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mitochondrial distribution and microtubule organization were examined in porcine oocytes after parthenogenesis, fertilization and somatic cell nuclear transfer (SCNT). Our results revealed that mitochondria are translocated from the oocyte's cortex to the perinuclear area by microtubules that either constitute the sperm aster in in vitro-fertilized (IVF) oocytes or originate from the donor cell centrosomes in SCNT oocytes. The ability to translocate mitochondria to the perinuclear area was lower in SCNT oocytes than in IVF oocytes. Sperm-induced activation rather than electrical activation of SCNT oocytes as well as the presence of the oocyte spindle enhanced perinuclear mitochondrial association with reconstructed nuclei, while removal of the oocyte spindle prior to sperm penetration decreased mitochondrial association with male pronuclei without having an apparent effect on microtubules. We conclude that factors derived from spermatozoa and oocyte spindles may affect the ability of zygotic microtubules to translocate mitochondria after IVF and SCNT in porcine oocytes. Mitochondrial association with pronuclei was positively related with embryo development after IVF. The reduced mitochondrial association with nuclei in SCNT oocytes may be one of the reasons for the low cloning efficiency which could be corrected by adding yet to be identified, sperm-derived factors that are normally present during physiological fertilization.
Authors:
Mika Katayama; Zhisheng Zhong; Liangxue Lai; Peter Sutovsky; Randall S Prather; Heide Schatten
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-07-28
Journal Detail:
Title:  Developmental biology     Volume:  299     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-18     Completed Date:  2006-11-29     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  206-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / drug effects,  metabolism
Electric Stimulation
Embryo, Mammalian / cytology,  drug effects,  metabolism*
Female
Fertilization in Vitro / methods*
Fetus / cytology,  drug effects
Fibroblasts / cytology,  drug effects
Hydrogen-Ion Concentration
Male
Microtubules / drug effects,  metabolism*
Mitochondria / drug effects,  metabolism*
Nocodazole / pharmacology
Nuclear Transfer Techniques
Oocytes / cytology,  drug effects
Research Embryo Creation / methods*
Sperm-Ovum Interactions
Spindle Apparatus / drug effects,  metabolism
Swine
Grant Support
ID/Acronym/Agency:
R01-RR13438-07/RR/NCRR NIH HHS; R03 HD043829/HD/NICHD NIH HHS; R03 HD043829-02/HD/NICHD NIH HHS; R03-HD43829-02/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
SH1WY3R615/Nocodazole
Comments/Corrections

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