| Mitochondrial damage and DNA depletion in cord blood and umbilical cord from infants exposed in utero to Combivir. | |
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MedLine Citation:
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PMID: 15096804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Although most uninfected infants born to women infected with HIV-1 show no clinical evidence of mitochondrial compromise, mitochondrial dysfunction has been reported in children born to women receiving zidovudine and/or lamivudine during pregnancy. In this pilot study we examined mitochondrial integrity in HIV-1-uninfected infants born to HIV-1-infected women receiving Combivir during pregnancy. DESIGN:: Samples of umbilical cord and cord blood were obtained from HIV-1-uninfected infants born to either HIV-1-infected women receiving Combivir therapy during pregnancy (n = 10) or HIV-1-uninfected women (n = 9). METHODS: Mitochondrial morphological integrity was examined in umbilical cords (n = 16) by electron microscopy and mtDNA quantity was determined in DNA from cord blood (n = 18) and umbilical cord (n = 18) by PCR-chemiluminescence immunoassay detection. RESULTS: In umbilical cords from six of nine infants born to HIV-1-infected mothers taking Combivir moderate to severe mitochondrial morphological damage was observed (P = 0.011), while none of seven unexposed infants showed similar damage. Compared to unexposed infants, statistically significant mtDNA depletion was observed in umbilical cord (P = 0.006) and cord blood (P = 0.003) from drug-exposed infants. CONCLUSIONS: A cohort of HIV-1-uninfected Combivir-exposed infants with no clinical symptoms showed morphological and molecular evidence of mitochondrial damage. |
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Authors:
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Rao L Divi; Vernon E Walker; Nancy A Wade; Kunio Nagashima; Steven K Seilkop; Mary Ellen Adams; Carol J Nesel; J Patrick O'Neill; Elaine J Abrams; Miriam C Poirier |
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Publication Detail:
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Type: Journal Article; Multicenter Study; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: AIDS (London, England) Volume: 18 ISSN: 0269-9370 ISO Abbreviation: AIDS Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-04-20 Completed Date: 2004-08-03 Revised Date: 2009-10-02 |
Medline Journal Info:
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Nlm Unique ID: 8710219 Medline TA: AIDS Country: England |
Other Details:
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Languages: eng Pagination: 1013-21 Citation Subset: IM; X |
Affiliation:
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CDI Section, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-HIV Agents
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adverse effects* CD4 Lymphocyte Count DNA, Mitochondrial / analysis*, drug effects Drug Combinations Female Fetal Blood / metabolism HIV Infections / drug therapy HIV-1 / isolation & purification Humans Infant, Newborn Lamivudine / adverse effects* Maternal-Fetal Exchange* Microscopy, Electron Mitochondria / drug effects*, ultrastructure Pilot Projects Pregnancy Pregnancy Complications, Infectious / drug therapy Prenatal Exposure Delayed Effects Umbilical Cord / metabolism, ultrastructure Zidovudine / adverse effects* |
| Grant Support | |
ID/Acronym/Agency:
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R01-HD33648/HD/NICHD NIH HHS; R01-HL72727/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anti-HIV Agents; 0/DNA, Mitochondrial; 0/Drug Combinations; 0/lamivudine, zidovudine drug combination; 134678-17-4/Lamivudine; 30516-87-1/Zidovudine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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