Document Detail


Mitochondrial creatine kinase activity and phosphate shuttling are acutely regulated by exercise in human skeletal muscle.
MedLine Citation:
PMID:  22907058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Energy transfer between mitochondrial and cytosolic compartments is predominantly achieved by creatine-dependent phosphate shuttling (PCr/Cr) involving mitochondrial creatine kinase (miCK). However, ADP/ATP diffusion through adenine nucleotide translocase (ANT) and voltage-dependent anion carriers (VDACs) is also involved in this process. To determine if exercise alters the regulation of this system, ADP-stimulated mitochondrial respiratory kinetics were assessed in permeabilized muscle fibre bundles (PmFBs) taken from biopsies before and after 2 h of cycling exercise (60% ) in nine lean males. Concentrations of creatine (Cr) and phosphocreatine (PCr) as well as the contractile state of PmFBs were manipulated in situ. In the absence of contractile signals (relaxed PmFBs) and miCK activity (no Cr), post-exercise respiratory sensitivity to ADP was reduced in situ (up to 126% higher apparent K(m) to ADP) suggesting inhibition of ADP/ATP diffusion between matrix and cytosolic compartments (possibly ANT and VDACs). However this effect was masked in the presence of saturating Cr (no effect of exercise on ADP sensitivity). Given that the role of ANT is thought to be independent of Cr, these findings suggest ADP/ATP, but not PCr/Cr, cycling through the outer mitochondrial membrane (VDACs) may be attenuated in resting muscle after exercise. In contrast, in contracted PmFBs, post-exercise respiratory sensitivity to ADP increased with miCK activation (saturating Cr; 33% lower apparent K(m) to ADP), suggesting prior exercise increases miCK sensitivity in situ. These observations demonstrate that exercise increases miCK-dependent respiratory sensitivity to ADP, promoting mitochondrial-cytosolic energy exchange via PCr/Cr cycling, possibly through VDACs. This effect may mask an underlying inhibition of Cr-independent ADP/ATP diffusion. This enhanced regulation of miCK-dependent phosphate shuttling may improve energy homeostasis through more efficient coupling of oxidative phosphorylation to perturbations in cellular energy charge during subsequent bouts of contraction.
Authors:
Christopher G R Perry; Daniel A Kane; Eric A F Herbst; Kazutaka Mukai; Daniel S Lark; David C Wright; George J F Heigenhauser; P Darrell Neufer; Lawrence L Spriet; Graham P Holloway
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-20
Journal Detail:
Title:  The Journal of physiology     Volume:  590     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-02     Completed Date:  2013-03-25     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  5475-86     Citation Subset:  IM    
Affiliation:
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada. cperry@yorku.ca
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / physiology*
Animals
Creatine Kinase, Mitochondrial Form / physiology*
Exercise / physiology*
Humans
Male
Muscle Contraction
Muscle, Skeletal / physiology*
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
R01 DK074825/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
58-64-0/Adenosine Diphosphate; EC 2.7.3.2/Creatine Kinase, Mitochondrial Form
Comments/Corrections

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