Document Detail


Mitochondrial correlation microscopy and nanolaser spectroscopy - new tools for biophotonic detection of cancer in single cells.
MedLine Citation:
PMID:  16292878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Currently, pathologists rely on labor-intensive microscopic examination of tumor cells using century-old staining methods that can give false readings. Emerging BioMicroNano-technologies have the potential to provide accurate, realtime, high-throughput screening of tumor cells without the need for time-consuming sample preparation. These rapid, nano-optical techniques may play an important role in advancing early detection, diagnosis, and treatment of disease. In this report, we show that laser scanning confocal microscopy can be used to identify a previously unknown property of certain cancer cells that distinguishes them, with single-cell resolution, from closely related normal cells. This property is the correlation of light scattering and the spatial organization of mitochondria. In normal liver cells, mitochondria are highly organized within the cytoplasm and highly scattering, yielding a highly correlated signal. In cancer cells, mitochondria are more chaotically organized and poorly scattering. These differences correlate with important bioenergetic disturbances that are hallmarks of many types of cancer. In addition, we review recent work that exploits the new technology of nanolaser spectroscopy using the biocavity laser to characterize the unique spectral signatures of normal and transformed cells. These optical methods represent powerful new tools that hold promise for detecting cancer at an early stage and may help to limit delays in diagnosis and treatment.
Authors:
Paul L Gourley; Judy K Hendricks; Anthony E McDonald; R Guild Copeland; Keith E Barrett; Cheryl R Gourley; Keshav K Singh; Robert K Naviaux
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review    
Journal Detail:
Title:  Technology in cancer research & treatment     Volume:  4     ISSN:  1533-0346     ISO Abbreviation:  Technol. Cancer Res. Treat.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-18     Completed Date:  2006-02-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101140941     Medline TA:  Technol Cancer Res Treat     Country:  United States    
Other Details:
Languages:  eng     Pagination:  585-92     Citation Subset:  IM    
Affiliation:
Biomolecular Interfaces and Systems, Sandia National Laboratories, Albuquerque, NM 87185, USA. plgourl@sandia.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Diagnostic Imaging / methods
Humans
Lasers
Microscopy, Confocal / methods*
Mitochondria / pathology*
Nanotechnology
Neoplasms / diagnosis*,  therapy
Grant Support
ID/Acronym/Agency:
R01-097714//PHS HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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