Document Detail


Mitochondrial copper metabolism in yeast: mutational analysis of Sco1p involved in the biogenesis of cytochrome c oxidase.
MedLine Citation:
PMID:  10079328     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Saccharomyces cerevisiae Sco1p is believed to be involved in the transfer of copper from the carrier Cox17p to the mitochondrial cytochrome c oxidase subunits 1 and 2. We here report on the results of a mutational analysis of Sco1p. The two cysteine residues of a potential metal-binding motif (CxxxC) are essential for protein function as shown by their substitution by alanines. Chimeras consisting of Sco1p and its homolog S. cerevisiae Sco2p restrict the specificity of Sco1p function to the N-terminal half of the protein. A candidate region for conferring specificity on Sco1p is a stretch of hydrophobic amino acids, which act as a membrane anchor. In line with this suggestion is the result that alterations of individual amino acids within this region impair Sco1p function.
Authors:
A Rentzsch; G Krummeck-Weiss; A Hofer; A Bartuschka; K Ostermann; G Rödel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current genetics     Volume:  35     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-06-16     Completed Date:  1999-06-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-8     Citation Subset:  IM    
Affiliation:
Institut für Genetik, Technische Universität Dresden, Mommsenstrasse 13, D-01062 Dresden, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Binding Sites / genetics
Copper / metabolism*
Electron Transport Complex IV / biosynthesis
Fungal Proteins / genetics
Membrane Proteins / genetics,  metabolism
Mitochondria / metabolism*
Mitochondrial Proteins
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Recombinant Fusion Proteins / genetics
Saccharomyces cerevisiae / genetics,  metabolism*
Saccharomyces cerevisiae Proteins*
Sequence Deletion
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Membrane Proteins; 0/Mitochondrial Proteins; 0/Recombinant Fusion Proteins; 0/SCO1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 7440-50-8/Copper; EC 1.9.3.1/Electron Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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