Document Detail

Mitochondrial content reflects oocyte variability and fertilization outcome.
MedLine Citation:
PMID:  16500323     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine the content of mitochondrial DNA (mtDNA) in oocytes from a range of patients with fertilization success and failure. DESIGN: Analysis of mtDNA content in fertilized and unfertilized oocytes and embryos by real-time polymerase chain reaction (PCR). SETTING: University hospital infertility and research center. PATIENT(S): Fifty-four women seeking treatment for infertility. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): A total of 142 fertilized and unfertilized oocytes were classified into three main groups. Group I consisted of 35 fertilized oocytes from 21 patients; group II, 65 unfertilized oocytes from 36 patients; and group III, 42 degenerate oocytes from 23 patients. Mitochondrial DNA content was determined by SYBR Green real-time PCR-based assay. RESULT(S): The mean mtDNA copy number for the fertilized oocytes was 250,454, whereas for the unfertilized group it was 163,698. There were significant differences for mtDNA copy number between the male factor and female factor infertility unfertilized oocytes and between the unexplained infertility and female factor infertility groups. The mean copy number for the degenerate oocyte group was 44,629, which was significantly different from the other subdivisions in this group. CONCLUSION(S): Mitochondrial DNA content is critical to fertilization outcome and serves as an important marker of oocyte quality, explaining some cases of fertilization failure.
Teresa Almeida Santos; Shahy El Shourbagy; Justin C St John
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fertility and sterility     Volume:  85     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-27     Completed Date:  2006-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  584-91     Citation Subset:  IM    
Departamento de Medicina Materno-fetal, Genética e Reprodução Humana, Hospitais da Universidade de Coimbra, Faculdade de Medicina, Coimbra, Portugal.
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MeSH Terms
Computer Systems
DNA, Mitochondrial / metabolism*
Fertilization / physiology*
Gene Dosage
Infertility, Female / genetics,  metabolism,  physiopathology*
Infertility, Male / genetics,  metabolism,  physiopathology*
Oocytes / metabolism*
Polymerase Chain Reaction
Reg. No./Substance:
0/DNA, Mitochondrial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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