Document Detail

Mitochondrial comparative proteomics of human ovarian cancer cells and their platinum-resistant sublines.
MedLine Citation:
PMID:  20957754     Owner:  NLM     Status:  MEDLINE    
Resistance to platinum-based chemotherapy is the major obstacle to successful treatment of ovarian cancer. It is evident that mitochondrial defects and the dysfunctions of oxidative phosphorylation and energy production in ovarian cancer cells were directly related to their resistance to platinum drugs. Using 2-D DIGE, we compared mitochondrial proteins from two platinum-sensitive human ovarian cancer cell lines (SKOV3 and A2780) with that of four platinum-resistant sublines (SKOV3/CDDP, SKOV3/CBP, A2780/CDDP, and A2780/CBP). Among the 236 differentially expressed spots, five mitochondrial proteins (ATP-α, PRDX3, PHB, ETF, and ALDH) that participate in the electron transport respiratory chain were identified through mass spectrometry. All of them are downregulated in one or two of the platinum-resistant cell lines. Three proteins (ATP-α, PRDX3, and PHB) were validated by using western blot and immunohistochemistry. There is a significant decrease of PHB in tumor tissues from ovarian cancer patients who were resistant to platinum-based chemotherapies. This is the first direct mitochondrial proteomic comparison between platinum-sensitive and resistant ovarian cancer cells. These studies demonstrated that 2-D DIGE-based proteomic analysis could be a powerful tool to investigate limited mitochondrial proteins, and the association of PHB expression with platinum resistance indicates that mitochondria defects may contribute to platinum resistance in ovarian cancer cells.
Zhiqin Dai; Jie Yin; Haojie He; Wenrui Li; Chunmei Hou; Xiaohong Qian; Ning Mao; Lingya Pan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  10     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-28     Completed Date:  2011-02-28     Revised Date:  2011-03-24    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3789-99     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.
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MeSH Terms
Blotting, Western
Cell Line, Tumor
Drug Resistance, Neoplasm
Electrophoresis, Gel, Two-Dimensional
Mitochondria / chemistry
Mitochondrial Proteins / chemistry,  metabolism*
Organoplatinum Compounds / pharmacology*
Ovarian Neoplasms / drug therapy,  metabolism*
Peroxiredoxins / chemistry,  metabolism
Proteomics / methods*
Repressor Proteins / chemistry,  metabolism
Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Reg. No./Substance:
0/Mitochondrial Proteins; 0/Organoplatinum Compounds; 0/Repressor Proteins; 0/prohibitin; EC protein, human; EC
Erratum In:
Proteomics. 2011 Mar;11(5):1012

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