| Mitochondrial biogenesis and mitochondrial activity during the progression of the cell cycle of human leukemic cells. | |
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MedLine Citation:
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PMID: 3409975 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondrial (mt) biogenesis and mt function were investigated during the cell cycle of leukemic cells. The study shows that the activity of enzymes involved in oxidative phosphorylation increases in the early G1 phase. This increase in activity precedes that of other mt enzymes such as citrate synthase and adenylate kinase. Therefore, the synthesis of mt enzymes, needed for the reduplication of the mt mass in the course of the cell cycle, occurs in a sequential order. The enzymes of the system for oxidative phosphorylation are composed of several subunits. Some of these subunits are encoded on mtDNA and synthesized by mt-specific RNA and protein synthesis. This explains why inhibition of mt protein synthesis during the progression of the cell cycle of G1-enriched cells results in an increasing shortage of ATP. This lack of ATP results first in progression delay and, subsequently, in a cell cycle block in early G1. Furthermore, shortage of ATP impairs the increase in activity of at least one mt matrix enzyme. This study offers new information about a number of aspects of mt biogenesis and mt function during cell cycle progression and elucidates the cytostatic mechanism resulting from prolonged inhibition of mt protein synthesis. |
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Authors:
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C Van den Bogert; P Muus; C Haanen; A Pennings; T E Melis; A M Kroon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 178 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1988-10-07 Completed Date: 1988-10-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 143-53 Citation Subset: IM |
Affiliation:
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Laboratory of Physiological Chemistry, Medical School, State University, Groningen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism Adenosine Triphosphate / metabolism Cell Cycle Doxycycline / pharmacology Humans Mitochondria / enzymology, physiology* Protein Biosynthesis Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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56-65-5/Adenosine Triphosphate; 564-25-0/Doxycycline; 58-64-0/Adenosine Diphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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