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Mitochondrial biogenesis and angiogenesis in skeletal muscle of the elderly.
MedLine Citation:
PMID:  21504786     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of this study was to test the hypotheses that 1) skeletal muscles of elderly subjects can adapt to a single endurance exercise bout and 2) endurance trained elderly subjects have higher expression/activity of oxidative and angiogenic proteins in skeletal muscle than untrained elderly people. To investigate this, lifelong endurance trained elderly (ET; n=8) aged 71.3±3.4years and untrained elderly subjects (UT; n=7) aged 71.3±4years, performed a cycling exercise bout at 75% VO(2max) with vastus lateralis muscle biopsies obtained before (Pre), immediately after exercise and at 2h of recovery. Capillarization was detected histochemically and oxidative enzyme activities were determined on isolated mitochondria. GLUT4, HKII, Cyt c and VEGF protein expression was measured on muscle lysates from Pre-biopsies, phosphorylation of AMPK and P38 on lysates from Pre and 0' biopsies, while PGC-1α, VEGF, HKII and TFAM mRNA content was determined at all time points. ET had ~30% higher PDH, CS, SDH, α-KG-DH and ATP synthase activities and ~20% higher capillarization than UT, reflecting increased skeletal muscle oxidative capacity with lifelong endurance exercise training. In addition, acute exercise increased PGC-1α mRNA 11-fold and VEGF mRNA 4-fold at 2h of recovery in UT, and AMPK phosphorylation ~5-fold immediately after exercise, relative to Pre, indicating an ability to adapt metabolically and angiogenically to endurance exercise. However, in ET PGC-1α mRNA only increased 5 fold and AMPK phosphorylation ~2-fold, while VEGF mRNA remained unchanged after the acute exercise bout. P38 increased similarly in ET and UT after exercise. In conclusion, the present findings suggest that lifelong endurance exercise training ensures an improved oxidative capacity of skeletal muscle, and that skeletal muscle of elderly subjects maintains the ability to respond to acute exercise.
Authors:
Ninna Iversen; Peter Krustrup; Hans N Rasmussen; Ulla F Rasmussen; Bengt Saltin; Henriette Pilegaard
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-13
Journal Detail:
Title:  Experimental gerontology     Volume:  -     ISSN:  1873-6815     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0047061     Medline TA:  Exp Gerontol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Affiliation:
Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark; Centre of Inflammation and Metabolism, University of Copenhagen, Copenhagen, Denmark; August Krogh Building, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
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