| Mitochondrial biogenesis and angiogenesis in skeletal muscle of the elderly. | |
| | |
MedLine Citation:
|
PMID: 21504786 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The aim of this study was to test the hypotheses that 1) skeletal muscles of elderly subjects can adapt to a single endurance exercise bout and 2) endurance trained elderly subjects have higher expression/activity of oxidative and angiogenic proteins in skeletal muscle than untrained elderly people. To investigate this, lifelong endurance trained elderly (ET; n=8) aged 71.3±3.4years and untrained elderly subjects (UT; n=7) aged 71.3±4years, performed a cycling exercise bout at 75% VO(2max) with vastus lateralis muscle biopsies obtained before (Pre), immediately after exercise and at 2h of recovery. Capillarization was detected histochemically and oxidative enzyme activities were determined on isolated mitochondria. GLUT4, HKII, Cyt c and VEGF protein expression was measured on muscle lysates from Pre-biopsies, phosphorylation of AMPK and P38 on lysates from Pre and 0' biopsies, while PGC-1α, VEGF, HKII and TFAM mRNA content was determined at all time points. ET had ~30% higher PDH, CS, SDH, α-KG-DH and ATP synthase activities and ~20% higher capillarization than UT, reflecting increased skeletal muscle oxidative capacity with lifelong endurance exercise training. In addition, acute exercise increased PGC-1α mRNA 11-fold and VEGF mRNA 4-fold at 2h of recovery in UT, and AMPK phosphorylation ~5-fold immediately after exercise, relative to Pre, indicating an ability to adapt metabolically and angiogenically to endurance exercise. However, in ET PGC-1α mRNA only increased 5 fold and AMPK phosphorylation ~2-fold, while VEGF mRNA remained unchanged after the acute exercise bout. P38 increased similarly in ET and UT after exercise. In conclusion, the present findings suggest that lifelong endurance exercise training ensures an improved oxidative capacity of skeletal muscle, and that skeletal muscle of elderly subjects maintains the ability to respond to acute exercise. |
| | |
Authors:
|
Ninna Iversen; Peter Krustrup; Hans N Rasmussen; Ulla F Rasmussen; Bengt Saltin; Henriette Pilegaard |
Related Documents
:
|
20345416 - The future: genes, physical activity and health. 18657996 - Arranging appropriate activities immigrant women's ideas of enabling exercise. 7167736 - The effect of a standardized work load on 'liver tests' in patients with chronic active... 16575256 - Prolonged seated immobility-associated venous coagulability in a factor v leiden hetero... 21252216 - Resistance and aerobic exercise protects against acute endothelial impairment induced b... 8226506 - Effects of dehydration on thermoregulatory responses of horses during low-intensity exe... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-4-13 |
Journal Detail:
|
Title: Experimental gerontology Volume: - ISSN: 1873-6815 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-4-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
|
Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark; Centre of Inflammation and Metabolism, University of Copenhagen, Copenhagen, Denmark; August Krogh Building, Department of Biology, University of Copenhagen, Copenhagen, Denmark. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Peripheral site ligand conjugation to a non-quaternary oxime enhances reactivation of nerve agent-in...
Next Document: Organic solvent adaptation of Gram positive bacteria: Applications and biotechnological potentials.