| Mitochondrial bioenergetics linked to the manifestation of programmed cell death during somatic embryogenesis of Abies alba. | |
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MedLine Citation:
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PMID: 19834734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present work reports changes in bioenergetic parameters and mitochondrial activities during the manifestation of two events of programmed cell death (PCD), linked to Abies alba somatic embryogenesis. PCD, evidenced by in situ nuclear DNA fragmentation (TUNEL assay), DNA laddering and cytochrome c release, was decreased in maturing embryogenic tissue with respect to the proliferation stage. In addition, the major cellular energetic metabolites (ATP, NAD(P)H and glucose-6-phosphate) were highered during maturation. The main mitochondrial activities changed during two developmental stages. Mitochondria, isolated from maturing, with respect to proliferating cell masses, showed an increased activity of the alternative oxidase, external NADH dehydrogenase and fatty-acid mediated uncoupling. Conversely, a significant decrease of the mitochondrial K (ATP)(+) channel activity was observed. These results suggest a correlation between mitochondrial activities and the manifestation of PCD during the development of somatic embryos. In particular, it is suggested that the K (ATP)(+) channel activity could induce an entry of K(+) into the matrix, followed by swelling and a release of cytochrome c during proliferation, whereas the alternative pathways, acting as anti-apoptotic factors, may partially counteract PCD events occurring during maturation of somatic embryos. |
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Authors:
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Elisa Petrussa; Alberto Bertolini; Valentino Casolo; Jana Krajn?kov?; Francesco Macr?; Angelo Vianello |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-16 |
Journal Detail:
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Title: Planta Volume: 231 ISSN: 1432-2048 ISO Abbreviation: Planta Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-01-21 Completed Date: 2010-03-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
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Languages: eng Pagination: 93-107 Citation Subset: IM |
Affiliation:
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Section of Plant Biology, Department of Biology and Plant Protection, University of Udine, via delle Scienze 91, 33100, Udine, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abies
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cytology*,
drug effects,
embryology*,
metabolism Adenosine Triphosphate / metabolism Apoptosis* / drug effects Cell Respiration / drug effects Cytochromes c / metabolism DNA Fragmentation / drug effects Embryonic Development* / drug effects Energy Metabolism* / drug effects Fatty Acids / pharmacology Glucose-6-Phosphate / metabolism Membrane Potential, Mitochondrial / drug effects Mitochondria / drug effects, enzymology, metabolism* NADP / metabolism Oxidoreductases / metabolism Oxygen / metabolism Potassium Channels / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Potassium Channels; 0/mitochondrial K(ATP) channel; 53-59-8/NADP; 56-65-5/Adenosine Triphosphate; 56-73-5/Glucose-6-Phosphate; 7782-44-7/Oxygen; 9007-43-6/Cytochromes c; EC 1.-/Oxidoreductases; EC 1.-/alternative oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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