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Mitochondrial autonomy. Sialic acid residues on the surface of isolated rat cerebral cortex and liver mitochondria.
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MedLine Citation:
PMID:  4653414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
N-acetylneuraminic acid at the surfaces of rat cerebral cortex and liver mitochondria and derived mitoplasts (inner membrane plus matrix particles) was studied biochemically and electrokinetically. Rat cerebral cortex mitochondria in 0.0145 M NaCl, 4.5% sorbitol, pH 7.2 +/- 0.1, 0.6 mM NaHCO(3), had an electrophoretic mobility of - 2.88 +/- 0.01 micro/sec per v per cm. In the same solution the electrophoretic mobility of rat liver mitochondria was - 2.01 +/- 0.02, of rat liver mitoplasts was - 1.22 +/- 0.07, and of rat cerebral cortex mitoplasts - 0.91 +/- 0.04 micro/sec per v per cm. Treatment of these particles with 50 microg neuraminidase/mg particle protein resulted in the following electrophoretic mobilities in micro/sec per v per cm: rat cerebral cortex mitochondria, - 2.27; rat liver mitochondria, - 1.40; rat cerebral cortex mitoplasts, - 0.78; and rat liver mitoplasts, - 1.10. Rat liver mitochondria, mitoplasts, and outer mitochondrial membranes contained 2.0, 1.1, and 4.1 nmoles of sialic acid/mg protein, respectively. 10% of the liver mitochondrial protein and 27.5% of the sialic acid was solubilized in the mitoplast and outer membrane isolation procedure. Rat cerebral cortex mitochondria, mitoplasts, and outer mitochondrial membranes contained 3.1, 0.8, and 6.2 nmoles sialic acid/mg protein, respectively; 10% of the brain mitochondrial protein and 49 % of the sialic acid was solubilized in the mitoplast and outer membrane isolation solution procedure. Treatment of both the rat liver and cerebral cortex mitochondria with 50 microg neuraminidase (dry weight) /mg protein resulted in the release of about 50% of the available outer membrane sialic acid residues. Treatment of all of the particles with trypsin caused release of sialic acid but did not greatly affect the particle electrophoretic mobility. In each instance, curves of pH vs. electrophoretic mobility indicated that the particle surface contained an acid dissociable group, most likely a carboxyl group of sialic acid with pK(a) approximately 2.7. Treatment of either the rat liver or the cerebral cortex mitochondria with trypsinized concanavalin A did not affect the particle electrophoretic mobility but did cause a decrease in the electrophoretic mobility of L5178Y mouse leukemic cells.
Authors:
H B Bosmann; M W Myers; D Dehond; R Ball; K R Case
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  55     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1972 Oct 
Date Detail:
Created Date:  1973-04-19     Completed Date:  1973-04-19     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  147-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fractionation
Cell Line
Cerebral Cortex / analysis,  cytology*
Concanavalin A / pharmacology
Electrophoresis
Hydrogen-Ion Concentration
Leukemia, Experimental
Membranes / analysis,  drug effects
Mice
Mitochondria / analysis*,  drug effects
Mitochondria, Liver / analysis*,  drug effects
Nerve Tissue Proteins / analysis
Neuraminic Acids / analysis*
Neuraminidase / pharmacology
Proteins / analysis
Rats
Trypsin / pharmacology
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Neuraminic Acids; 0/Proteins; 11028-71-0/Concanavalin A; EC 3.2.1.18/Neuraminidase; EC 3.4.21.4/Trypsin
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1972 by The Rockefeller University Press
Received Day: 28 Month: 3 Year: 1972
Revision Received Day: 12 Month: 6 Year: 1972
Print publication date: Day: 1 Month: 10 Year: 1972
Volume: 55 Issue: 1
First Page: 147 Last Page: 160
ID: 2108747
PubMed Id: 4653414

MITOCHONDRIAL AUTONOMY : Sialic Acid Residues on the Surface of Isolated Rat Cerebral Cortex and Liver Mitochondria
H. Bruce Bosmann
Marjorie W. Myers
Delena Dehond
Richard Ball
Kenneth R. Case
From the Department of Pharmacology and Toxicology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642


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