Document Detail


Mitochondrial aspartate aminotransferase by immunoprecipitation method in patients with acute myocardial infarction.
MedLine Citation:
PMID:  2805563     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The semiautomated kinetic procedure for determining cytoplasmic aspartate aminotransferase (c-AST) and mitochondrial aspartate aminotransferase (m-AST) activities was studied by the use of the immunoprecipitation method with anti-c-AST antibody in serum samples. The measured activity for m-AST remained constant after the addition of c-AST up to 1,000 IU/l throughout the 60-min incubation period. The measurements of m-AST activity were reproducible, selective and complete as determined by a purified m-AST. The precision of this method was as good as that of the manual method (CV 2.04%). The present method and the manual method gave approximately equal results for m-AST (r = 0.987). The effects of activations on m- and c-AST activity were compared by the addition of pyridoxal 5'-phosphate to sera of various diseases. A higher activation ratio by pyridoxal 5'-phosphate was observed on both aspartate aminotransferase activities in the serum of patients with ischemic heart diseases than in the serum of patients with liver diseases.
Authors:
H Okabe; H Sugiuchi; N Yamane; N Watanabe; M Inoue; Y Morino; K Hayasaki; H Teranishi; S Inayama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical physiology and biochemistry     Volume:  7     ISSN:  0252-1164     ISO Abbreviation:  Clin Physiol Biochem     Publication Date:  1989  
Date Detail:
Created Date:  1989-12-01     Completed Date:  1989-12-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8305885     Medline TA:  Clin Physiol Biochem     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  109-18     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Aspartate Aminotransferases / metabolism*
Cytoplasm / enzymology
Enzyme Activation
Female
Humans
Kinetics
Male
Mitochondria / drug effects,  enzymology*
Myocardial Infarction / enzymology*
Precipitin Tests
Reference Values
Chemical
Reg. No./Substance:
EC 2.6.1.1/Aspartate Aminotransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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