Document Detail

Mitochondrial PO2 measured by delayed fluorescence of endogenous protoporphyrin IX.
MedLine Citation:
PMID:  17060918     Owner:  NLM     Status:  MEDLINE    
Molecular oxygen is the primary oxidant in biological systems. The ultimate destination of oxygen in vivo is the mitochondria where it is used in oxidative phosphorylation. The ability of this process to produce an amount of high-energy phosphates adequate to sustain life highly depends on the available amount of oxygen. Despite a vast array of techniques to measure oxygen, major (patho)physiological questions remain unanswered because of the unavailability of quantitative techniques to measure mitochondrial oxygen in situ. Here we demonstrate that mitochondrial PO(2) can be directly measured in living cells by harnessing the delayed fluorescence of endogenous protoporphyrin IX (PpIX), thereby providing a technique with the potential for a wide variety of applications. We applied this technique to different cell lines (V-79 Chinese hamster lung fibroblasts, HeLa cells and IMR 32-K1 neuroblastoma cells) and present the first direct measurements of the oxygen gradient between the mitochondria and the extracellular volume.
Egbert G Mik; Jan Stap; Michiel Sinaasappel; Johan F Beek; Jacob A Aten; Ton G van Leeuwen; Can Ince
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature methods     Volume:  3     ISSN:  1548-7091     ISO Abbreviation:  Nat. Methods     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-24     Completed Date:  2007-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  939-45     Citation Subset:  IM    
Department of Physiology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
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MeSH Terms
Cell Line
Cells, Cultured
Hela Cells
Microscopy, Fluorescence
Mitochondria / chemistry,  metabolism*
Oxygen / analysis,  metabolism*
Oxygen Consumption
Protoporphyrins / chemistry,  metabolism*
Sensitivity and Specificity
Reg. No./Substance:
0/Protoporphyrins; 553-12-8/protoporphyrin IX; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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