Document Detail


Mitochondrial heat shock protein-90 modulates vascular smooth muscle cell survival and the vascular injury response in vivo.
MedLine Citation:
PMID:  22841823     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The healing response of blood vessels from the vascular injury induced by therapeutic interventions is characterized by increased cellularity and tissue remodeling. Frequently, this leads to intimal hyperplasia and lumen narrowing, with significant clinical sequelae. Vascular smooth muscle cells are the primary cell type involved in this process, wherein they express a dedifferentiated phenotype that transiently resembles neoplastic transformation. Recent studies have highlighted the role of mitochondrial proteins, such as the molecular chaperone heat shock protein-90 (Hsp90), in promoting cancer cell survival, which leads to new candidate chemotherapeutic agents for neoplastic disease. Herein, we identify mitochondrial Hsp90 as a key modulator of the vascular injury response. Hsp90 expression is up-regulated in injured arteries and colocalizes with the apoptosis inhibitor, survivin, in vascular smooth muscle cell in vitro and in vivo. By using a proteomic approach, we demonstrate that targeted disruption of mitochondrial Hsp90 chaperone function in vascular smooth muscle cell leads to loss of cytoprotective client proteins (survivin and Akt), induces mitochondrial permeability, and leads to apoptotic cell death. Hsp90 targeting using a cell-permeable peptidomimetic agent resulted in marked attenuation of neointimal lesions in a murine arterial injury model. These findings suggest that mitochondrial Hsp90 chaperone function is an important regulator of intimal hyperplasia and may have implications for molecular strategies that promote the long-term patency of cardiovascular interventions.
Authors:
Andrew W Hoel; Peng Yu; Khanh P Nguyen; Xinxin Sui; Janet Plescia; Dario C Altieri; Michael S Conte
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-27
Journal Detail:
Title:  The American journal of pathology     Volume:  181     ISSN:  1525-2191     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-24     Completed Date:  2013-02-06     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1151-7     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2012 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
Affiliation:
Division of Vascular and Endovascular Surgery, Brigham and Women's Hospital, Boston, Massachusetts, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Blood Vessels / drug effects,  metabolism,  pathology*
Cell Survival / drug effects
Cytoprotection / drug effects
Gene Targeting
HSP90 Heat-Shock Proteins / metabolism*
Humans
Hyperplasia
Inhibitor of Apoptosis Proteins / metabolism
Male
Mice
Mice, Inbred C57BL
Mitochondria / drug effects,  metabolism*
Muscle, Smooth, Vascular / pathology*
Myocytes, Smooth Muscle / drug effects,  metabolism,  pathology*
Peptide Fragments / pharmacology
Rabbits
Tunica Intima / drug effects,  metabolism,  pathology
Grant Support
ID/Acronym/Agency:
HL085157/HL/NHLBI NIH HHS; R01 HL085157/HL/NHLBI NIH HHS; T32-HL007734-13/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/BIRC5 protein, human; 0/HSP90 Heat-Shock Proteins; 0/Inhibitor of Apoptosis Proteins; 0/Peptide Fragments; 0/shepherdin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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