| Mitochondrial DNA sequences are present inside nuclear DNA in rat tissues and increase with age. | |
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MedLine Citation:
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PMID: 20546951 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondrial DNA (mtDNA) mutations increase with age. However, the number of cells with predominantly mutated mtDNA is small in old animals. Here a new hypothesis is proposed: mtDNA fragments may insert into nuclear DNA contributing to aging and related diseases by alterations in the nucleus. Real-time PCR quantification shows that sequences of cytochrome oxidase III and 16S rRNA from mtDNA are present in highly purified nuclei from liver and brain in young and old rats. The sequences of these insertions revealed that they contain single nucleotide polymorphisms identical to those present in mtDNA of the same animal. Interestingly, the amount of mitochondrial sequences in nuclear DNA increases with age in both tissues. In situ hybridization of mtDNA to nuclear DNA confirms the presence of mtDNA sequences inside nuclear DNA in rat hepatocytes. Bone marrow metaphase cells from both young and old rats show mtDNA at centromeric regions in 20 out of the 2n=40 chromosomes. Consequently, mitochondria can be a major trigger of aging but the final target could also be the nucleus. |
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Authors:
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Pilar Caro; José Gómez; Alessandro Arduini; Mónica González-Sánchez; Miriam González-García; Consuelo Borrás; José Viña; María J Puertas; Juan Sastre; Gustavo Barja |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-28 |
Journal Detail:
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Title: Mitochondrion Volume: 10 ISSN: 1872-8278 ISO Abbreviation: Mitochondrion Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-09 Completed Date: 2010-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100968751 Medline TA: Mitochondrion Country: Netherlands |
Other Details:
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Languages: eng Pagination: 479-86 Citation Subset: IM |
Copyright Information:
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(c) 2010 Mitochondria Research Society. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Animal Physiology-II, Complutense University of Madrid, Spain. pcaromar@bio.ucm.es |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Brain / pathology Chromosomes* DNA, Mitochondrial / genetics*, isolation & purification* Electron Transport Complex IV / genetics In Situ Hybridization Liver / pathology Male Mutagenesis, Insertional* Polymorphism, Single Nucleotide RNA, Ribosomal, 16S / genetics Rats Rats, Wistar Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
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0/DNA, Mitochondrial; 0/RNA, Ribosomal, 16S; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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