Document Detail

Mitochondrial DNA from colorectal cancer cells promotes the malignant phenotype of NIH3T3 cells.
MedLine Citation:
PMID:  18595746     Owner:  NLM     Status:  MEDLINE    
We investigated the potential role of mitochondrial DNA (mtDNA) in colorectal carcinogenesis by constructing a eukaryotic expression vector of the mitochondrial D-loop gene from colorectal cancer cell SW480 and transfected NIH3T3 cells. The NIH3T3/SW480 cells exhibited a significantly increased growth rate and colony formation rate, and also had a decreased apoptotic rate. Polyploidy and aberrant chromosomes were detected in the NIH3T3/SW480 cells by chromosome karyotype analysis. Our results suggested that mtDNA from colorectal cancer cells promotes the malignant phenotype of NIH3T3 cells. Further study of the biological functions of NIH3T3/SW480 cells might be helpful in understanding the role of mtDNA in colorectal carcinogenesis.
Yan Li; Song Weibing; Hong Liu; Ji Hongli; Lai Zhuosheng; Wang Yadong; Xiao Bing; Fan Daiming
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-10
Journal Detail:
Title:  Cell biology international     Volume:  32     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-25     Completed Date:  2008-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  979-83     Citation Subset:  IM    
Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 692 Sheng Li Southern Road, Guangzhou, Guangdong Province 510515, PR China.
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MeSH Terms
Cell Line, Tumor
Cell Proliferation
Cell Transformation, Neoplastic*
Chromosome Aberrations
Colorectal Neoplasms / genetics*
DNA, Mitochondrial / metabolism*
Genetic Vectors
NIH 3T3 Cells
Reg. No./Substance:
0/DNA, Mitochondrial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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