Document Detail


Mitochondrial DNA damage analysis in bronchoalveolar lavage cells of preterm infants.
MedLine Citation:
PMID:  20036884     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In mechanically ventilated preterm infants, the combination of immaturity, volutrauma, oxidative stress, and inflammatory processes can lead to chronic lung injury. Mitochondrial DNA (mtDNA) is more susceptible to oxidative damage than nuclear DNA. We aimed to investigate the level of mtDNA damage (deletions, mutations and changes in copy number) in bronchoalveolar lavage (BAL) cells from 10 preterm infants (27-30 weeks). A first BAL (BAL1) was done within 24 h of endotracheal intubation and BAL2 was performed 30-103 h thereafter. Deletions were analyzed by long range PCR, point mutations by heteroduplex analysis of the D-loop region, and copy number changes by real-time PCR. Using these methods, no deletions were found in any of the BAL samples. When BAL1 and BAL2 samples were compared no new mutations were found. In contrast, a marked decrease in mtDNA copy number was observed in 5 patients. In conclusion, we found that exposure of preterm infants to short term mechanical ventilation did not lead to detrimental consequences for the mtDNA in the form of mutations or deletions.
Authors:
Bea Zoer; Jasper Valentijn Been; Eveline Jongen; Anne Debeer; Alexandra Hendrickx; Hubertus J Smeets; Luc Jacques Zimmermann; Eduardo Villamor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-01
Journal Detail:
Title:  Frontiers in bioscience (Elite edition)     Volume:  2     ISSN:  1945-0508     ISO Abbreviation:  Front Biosci (Elite Ed)     Publication Date:  2010  
Date Detail:
Created Date:  2009-12-28     Completed Date:  2010-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101485240     Medline TA:  Front Biosci (Elite Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  361-8     Citation Subset:  IM    
Affiliation:
Department of Pediatrics Maastricht University Medical Center, GROW School for Oncology and Developmental Biology, 6202 AZ Maastricht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Bronchoalveolar Lavage Fluid / cytology*
DNA Copy Number Variations / genetics
DNA Damage / genetics*
DNA Mutational Analysis
DNA Primers / genetics
DNA, Mitochondrial / genetics*
Humans
Infant, Newborn
Netherlands
Premature Birth / genetics*
Respiration, Artificial / adverse effects*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Mitochondrial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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