Document Detail


Mitochondria-derived reactive oxygen species mediate blue light-induced death of retinal pigment epithelial cells.
MedLine Citation:
PMID:  15191057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Throughout the lifetime of an individual, light is focused onto the retina. The resulting photooxidative stress can cause acute or chronic retinal damage. The pathogenesis of age-related macular degeneration (AMD), the leading cause of legal blindness in the developed world, involves oxidative stress and death of the retinal pigment epithelium (RPE) followed by death of the overlying photoreceptors. Evidence suggests that damage due to exposure to light plays a role in AMD and other age-related eye diseases. In this work a system for light-induced damage and death of the RPE, based on the human ARPE-19 cell line, was used. Induction of mitochondria-derived reactive oxygen species (ROS) is shown to play a critical role in the death of cells exposed to short-wavelength blue light (425 +/- 20 nm). ROS and cell death are blocked either by inhibiting the mitochondrial electron transport chain or by mitochondria-specific antioxidants. These results show that mitochondria are an important source of toxic oxygen radicals in blue light-exposed RPE cells and may indicate new approaches for treating AMD using mitochondria-targeted antioxidants.
Authors:
Ayala King; Eyal Gottlieb; David G Brooks; Michael P Murphy; Joshua L Dunaief
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  79     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-06-11     Completed Date:  2004-09-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  470-5     Citation Subset:  IM    
Affiliation:
F.M. Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Aging / radiation effects
Antioxidants / pharmacology
Apoptosis / drug effects,  radiation effects*
Cell Line
Electron Transport
Humans
Light*
Mitochondria / drug effects,  metabolism*,  radiation effects
Oxidative Stress / radiation effects
Pigment Epithelium of Eye / metabolism,  radiation effects*,  ultrastructure
Reactive Oxygen Species / antagonists & inhibitors,  metabolism*
Grant Support
ID/Acronym/Agency:
EY00417/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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