Document Detail


Mitochondria and apicoplast of Plasmodium falciparum: behaviour on subcellular fractionation and the implication.
MedLine Citation:
PMID:  17289446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques--Percoll density gradient centrifugation and fluorescence-activated organelle sorting--for improving the purity of mitochondria isolated from the crude organelle preparation of Plasmodium falciparum. To our surprise, the apicoplast was inseparable from the plasmodial mitochondrion by each method. This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium.
Authors:
Tamaki Kobayashi; Shigeharu Sato; Shinzaburo Takamiya; Kanako Komaki-Yasuda; Kazuhiko Yano; Ayami Hirata; Izumi Onitsuka; Masayuki Hata; Fumika Mi-ichi; Takeshi Tanaka; Toshiharu Hase; Atsushi Miyajima; Shin-ichiro Kawazu; Yoh-ichi Watanabe; Kiyoshi Kita
Related Documents :
15145636 - Serum lipopolysaccharide-binding protein prediction of severe bacterial infection in ci...
11279176 - Identification of an atp-binding cassette transporter for export of the o-antigen acros...
20109466 - Full-length recombinant plasmodium falciparum var2csa binds specifically to cspg and in...
24662306 - Piceatannol attenuates cardiac hypertrophy in an animal model through regulation of the...
9879066 - [3h]-bibp3226 and [3h]-npy binding to intact sk-n-mc cells and cho cells expressing the...
9246936 - Modulation of the insulin-like growth factor-binding proteins by follicle size in the h...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-09
Journal Detail:
Title:  Mitochondrion     Volume:  7     ISSN:  1567-7249     ISO Abbreviation:  Mitochondrion     Publication Date:    2007 Feb-Apr
Date Detail:
Created Date:  2007-03-12     Completed Date:  2007-07-11     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  100968751     Medline TA:  Mitochondrion     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  125-32     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fractionation
Centrifugation, Density Gradient
Microscopy, Electron
Mitochondria / ultrastructure*
Organelles / ultrastructure*
Plasmodium falciparum / ultrastructure*
Grant Support
ID/Acronym/Agency:
MC_U117532072//Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hybrid bioelectrocatalyst for hydrogen peroxide reduction: immobilization of enzyme within organic-i...
Next Document:  The i.v. infusion of mannitol decreases airway responsiveness to methacholine in asthma.