Document Detail

Mitochondria and apicoplast of Plasmodium falciparum: behaviour on subcellular fractionation and the implication.
MedLine Citation:
PMID:  17289446     Owner:  NLM     Status:  MEDLINE    
The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques--Percoll density gradient centrifugation and fluorescence-activated organelle sorting--for improving the purity of mitochondria isolated from the crude organelle preparation of Plasmodium falciparum. To our surprise, the apicoplast was inseparable from the plasmodial mitochondrion by each method. This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium.
Tamaki Kobayashi; Shigeharu Sato; Shinzaburo Takamiya; Kanako Komaki-Yasuda; Kazuhiko Yano; Ayami Hirata; Izumi Onitsuka; Masayuki Hata; Fumika Mi-ichi; Takeshi Tanaka; Toshiharu Hase; Atsushi Miyajima; Shin-ichiro Kawazu; Yoh-ichi Watanabe; Kiyoshi Kita
Related Documents :
2241896 - 5'-s-(2-aminoethyl)-n6-(4-nitrobenzyl)-5'-thioadenosine (saenta), a novel ligand with h...
1481986 - Polymyxin b-horseradish peroxidase conjugates as tools in endotoxin research.
8898966 - Physiological activation of the igh 3' enhancer in b lineage cells is not blocked by pa...
12748176 - Identification and characterization of heme-interacting proteins in the malaria parasit...
11686296 - Pseudo-phosphatase sbf1 contains an n-terminal gef homology domain that modulates its g...
16707926 - Identification and localization of heparin-binding region of snake venom vegf and its b...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-09
Journal Detail:
Title:  Mitochondrion     Volume:  7     ISSN:  1567-7249     ISO Abbreviation:  Mitochondrion     Publication Date:    2007 Feb-Apr
Date Detail:
Created Date:  2007-03-12     Completed Date:  2007-07-11     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  100968751     Medline TA:  Mitochondrion     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  125-32     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Fractionation
Centrifugation, Density Gradient
Microscopy, Electron
Mitochondria / ultrastructure*
Organelles / ultrastructure*
Plasmodium falciparum / ultrastructure*
Grant Support
MC_U117532072//Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Hybrid bioelectrocatalyst for hydrogen peroxide reduction: immobilization of enzyme within organic-i...
Next Document:  The i.v. infusion of mannitol decreases airway responsiveness to methacholine in asthma.