Document Detail


Mitochondria-targeted peptide accelerates ATP recovery and reduces ischemic kidney injury.
MedLine Citation:
PMID:  21546574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The burst of reactive oxygen species (ROS) during reperfusion of ischemic tissues can trigger the opening of the mitochondrial permeability transition (MPT) pore, resulting in mitochondrial depolarization, decreased ATP synthesis, and increased ROS production. Rapid recovery of ATP upon reperfusion is essential for survival of tubular cells, and inhibition of oxidative damage can limit inflammation. SS-31 is a mitochondria-targeted tetrapeptide that can scavenge mitochondrial ROS and inhibit MPT, suggesting that it may protect against ischemic renal injury. Here, in a rat model of ischemia-reperfusion (IR) injury, treatment with SS-31 protected mitochondrial structure and respiration during early reperfusion, accelerated recovery of ATP, reduced apoptosis and necrosis of tubular cells, and abrogated tubular dysfunction. In addition, SS-31 reduced medullary vascular congestion, decreased IR-mediated oxidative stress and the inflammatory response, and accelerated the proliferation of surviving tubular cells as early as 1 day after reperfusion. In summary, these results support MPT as an upstream target for pharmacologic intervention in IR injury and support early protection of mitochondrial function as a therapeutic maneuver to prevent tubular apoptosis and necrosis, reduce oxidative stress, and reduce inflammation. SS-31 holds promise for the prevention and treatment of acute kidney injury.
Authors:
Hazel H Szeto; Shaoyi Liu; Yi Soong; Dunli Wu; Shaun F Darrah; Feng-Ying Cheng; Zhihong Zhao; Michael Ganger; Clara Y Tow; Surya V Seshan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-05-05
Journal Detail:
Title:  Journal of the American Society of Nephrology : JASN     Volume:  22     ISSN:  1533-3450     ISO Abbreviation:  J. Am. Soc. Nephrol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-01     Completed Date:  2011-08-25     Revised Date:  2012-06-19    
Medline Journal Info:
Nlm Unique ID:  9013836     Medline TA:  J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1041-52     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Weill Cornell Medical College, New York, NY 10021, USA. hhszeto@med.cornell.edu
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MeSH Terms
Descriptor/Qualifier:
Acute Kidney Injury / etiology*,  physiopathology,  prevention & control*
Adenosine Triphosphate / metabolism*
Animals
Antioxidants / pharmacology,  therapeutic use*
Apoptosis / drug effects,  physiology
Kidney Tubules / drug effects,  physiology
Male
Mitochondria / drug effects,  physiology
Mitochondrial Membrane Transport Proteins / drug effects,  physiology
Models, Animal
Oligopeptides / pharmacology,  therapeutic use*
Oxidative Stress / drug effects,  physiology
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism
Regeneration / drug effects,  physiology
Reperfusion Injury / complications*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Mitochondrial Membrane Transport Proteins; 0/Oligopeptides; 0/Reactive Oxygen Species; 0/arginyl-2,'6'-dimethyltyrosyl-lysyl-phenylalaninamide; 0/mitochondrial permeability transition pore; 56-65-5/Adenosine Triphosphate
Comments/Corrections
Comment In:
J Am Soc Nephrol. 2011 Jun;22(6):986-9   [PMID:  21566050 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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