Document Detail


Mitigation of signal suppression caused by the use of trifluoroacetic acid in liquid chromatography mobile phases during liquid chromatography/mass spectrometry analysis via post-column addition of ammonium hydroxide.
MedLine Citation:
PMID:  22592995     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A method has been developed to reduce the mass spectrometric ion signal suppression associated with the use of TFA as an additive in LC mobile phases. Through post-column infusion of diluted NH(4) OH solution to LC eluents, the ammonium ion introduced causes the neutral analyte-TFA ion pair to dissociate which consequently releases the protonated analyte as free ions into the gas phase (through regular electrospray ionization mechanisms). An ion signal improvement from 1.2 to 20 times for a variety of compounds had been achieved through the application of this method. The molar ratios of NH(4) OH:TFA which result in a reduction of signal suppression were determined to be between 0.5:1 and 50:1. In addition, it was shown that this NH(4) OH infusion method could reduce the level of doubly-charged species and the product ions formed via in-source collision. The use of diluted NH(4) OH solution is favorable since it is compatible with mass spectrometry analysis, and it is applicable in both positive and negative-ion generation mode. Copyright © 2012 John Wiley & Sons, Ltd.
Authors:
Chang-Ching Chan; Mark S Bolgar; Dilusha Dalpathado; David K Lloyd
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  26     ISSN:  1097-0231     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1507-14     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Affiliation:
Bristol-Myers Squibb Analytical and Bioanalytical Development, New Brunswick, NJ, 08903, USA. chang-ching.chan@bms.com.
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