Document Detail

Mithramycin and DIPI: a pair of fluorochromes specific for GC-and AT-rich DNA respectively.
MedLine Citation:
PMID:  67077     Owner:  NLM     Status:  MEDLINE    
The AT specificity of the fluorochromes DIPI and DAPI and the GC specificity of mithramycin are evidenced by observations in human, mouse, and bovine chromosomes. DIPI and DAPI produce a pattern similar to Hoechst 33258 in all three species, whereas mithramycin results in a reverse pattern. The AT-rich centromeric heterochromatin in mouse is brilliantly stained by DIPI or DAPI and remains nearly invisible after mithramycin staining. In the GC-rich centromeric heterochromatin of cattle the opposite behavior is observed.
W Schnedl; M Breitenbach; G Stranzinger
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  36     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-06-22     Completed Date:  1977-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  299-305     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Fluorescent Dyes*
Staining and Labeling*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Heterochromatin; 18378-89-7/Plicamycin

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