Document Detail


Missense and splice site mutations in SPG4 suggest loss-of-function in dominant spastic paraplegia.
MedLine Citation:
PMID:  11985387     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied nine Italian families with a pure form of autosomal dominant spastic paraplegia (ADHSP) to assess the frequency of mutations in the SPG4 gene. We observed marked intrafamilial variability in both age-at-onset and clinical severity, ranging from severe congenital presentation to mild involvement after age 55 years to healthy carriers of the mutation after age 70. Four of nine probands harboured SPG4 mutations, We identified three new SPG4 mutations, all predicting a loss-of-func-tion with apparently important consequences for spastin function. RT-PCR studies predict loss-of-function as a possible mechanism leading to spastin-related HSP. The current study expands the spectrum of allelic variants in SPG4, confirming their pathological significance in pure AD-HSP and suggesting implications for the presumed function of spastin.
Authors:
Clarice Patrono; Carlo Casali; Alessandra Tessa; Federica Cricchi; Daniela Fortini; Rosalba Carrozzo; Gabriele Siciliano; Enrico Bertini; Filippo M Santorelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurology     Volume:  249     ISSN:  0340-5354     ISO Abbreviation:  J. Neurol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-05-02     Completed Date:  2002-10-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0423161     Medline TA:  J Neurol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  200-5     Citation Subset:  IM    
Affiliation:
IRCCS-Children's Hospital Bambino Gesù, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / genetics*,  metabolism
Adolescent
Adult
Age of Onset
Aged
Alternative Splicing / genetics*
Amino Acid Sequence / genetics
Child
Child, Preschool
Chromosomes, Human, Pair 2
DNA Mutational Analysis
Exons / genetics
Female
Gene Frequency / genetics
Genetic Testing
Humans
Infant
Italy
Male
Middle Aged
Mutation, Missense / genetics*
Pedigree
RNA, Messenger / genetics*
Spastic Paraplegia, Hereditary / genetics*,  physiopathology
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/SPAST protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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