| Misregulation of poly(ADP-ribose) polymerase-1 activity and cell type-specific loss of poly(ADP-ribose) synthesis in the cerebellum of aged rats. | |
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MedLine Citation:
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PMID: 15857403 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protein modification by ADP-ribose polymers is a common regulatory mechanism in eukaryotic cells and is involved in several aspects of brain physiology and physiopathology, including neurotransmission, memory formation, neurotoxicity, ageing and age-associated diseases. Here we show age-related misregulation of poly(ADP-ribose) synthesis in rat cerebellum as revealed by: (i) reduced poly(ADP-ribose) polymerase-1 (PARP-1) activation in response to enzymatic DNA cleavage, (ii) altered protein poly(ADP-ribosyl)ation profiles in isolated nuclei, and (iii) cell type-specific loss of poly(ADP-ribosyl)ation capacity in granule cell layer and Purkinje cells in vivo. In particular, although PARP-1 could be detected in virtually all granule cells, only a fraction of them appeared to be actively engaged in poly(ADP-ribose) synthesis and this fraction was reduced in old rat cerebellum. NAD(+), quantified in tissue homogenates, was essentially the same in the cerebellum of young and old rats suggesting that in vivo factors other than PARP-1 content and/or NAD(+) levels may be responsible for the age-associated lowering of poly(ADP-ribose) synthesis. Moreover, PARP-1 expression was substantially down-regulated in Purkinje cells of senescent rats. |
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Authors:
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M Malanga; M Romano; A Ferone; A Petrella; G Monti; R Jones; E Limatola; B Farina |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurochemistry Volume: 93 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-04-28 Completed Date: 2005-07-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 1000-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University Federico II, Naples, Italy. malanga@vetpharm.unizh.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism* Animals Blotting, Western / methods Brain / cytology, enzymology*, growth & development Brain Chemistry / physiology Cell Nucleus / enzymology Immunohistochemistry / methods Male NAD / metabolism Poly Adenosine Diphosphate Ribose / metabolism* Poly(ADP-ribose) Polymerases / metabolism* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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26656-46-2/Poly Adenosine Diphosphate Ribose; 53-84-9/NAD; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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