Document Detail

Mismatch repair in Xenopus egg extracts: DNA strand breaks act as signals rather than excision points.
MedLine Citation:
PMID:  8816768     Owner:  NLM     Status:  MEDLINE    
In Xenopus egg extracts, DNA strand breaks (nicks) located 3' or 5' to a mismatch cause an overall 3-fold stimulation of the repair of the mismatch in circular heteroduplex DNA molecules. The increase in mismatch repair is almost entirely due to an increase in repair of the nicked strand, which is stimulated 5-fold. Repair synthesis is centered to the mismatch site, decreases symmetrically on both sides, and its position is not significantly altered by the presence of the nick. Therefore, it appears that in the Xenopus germ cells, the mismatch repair system utilizes nicks as signals for the induction and direction of mismatch repair, but not as the start or end point for excision and resynthesis.
I Varlet; B Canard; P Brooks; G Cerovic; M Radman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  93     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-11-13     Completed Date:  1996-11-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10156-61     Citation Subset:  IM    
Laboratoire de Mutagenèse, Institut Jacques Mound, Centre National de la Recherche Scientifique, UMR 9922, Paris, France.
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MeSH Terms
Bacterial Proteins*
Base Composition
DNA / biosynthesis*
DNA Damage*
DNA Repair*
DNA, Circular / biosynthesis
Deoxyribonucleases, Type II Site-Specific
Nucleic Acid Heteroduplexes / biosynthesis*
Oocytes / physiology*
Restriction Mapping
Tissue Extracts
Xenopus laevis
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Circular; 0/Nucleic Acid Heteroduplexes; 0/Tissue Extracts; 9007-49-2/DNA; EC 3.1.21.-/endodeoxyribonuclease MluI; EC, Type II Site-Specific

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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