Document Detail


Mismatch and base excision repair proficiency in murine embryonic stem cells.
MedLine Citation:
PMID:  21315663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accumulation of mutations in embryonic stem (ES) cells would be detrimental to an embryo derived from these cells, and would adversely affect multiple organ systems and tissue types. ES cells have evolved multiple mechanisms to preserve genomic integrity that extend beyond those found in differentiated cell types. The present study queried whether mismatch repair (MMR) and base-excision repair (BER) may play a role in the maintenance of murine ES cell genomes. The MMR proteins Msh2 and Msh6 are highly elevated in mouse ES cells compared with mouse embryo fibroblasts (MEFs), as are Pms2 and Mlh1, albeit to a lesser extent. Cells transfected with an MMR reporter plasmid showed that MMR repair capacity is low in MEFs, but highly active in wildtype ES cells. As expected, an ES cell line defective in MMR was several-fold less effective in repair level than wildtype ES cells. Like proteins that participate in MMR, the level of proteins involved in BER was elevated in ES cells compared with MEFs. When BER activity was examined biochemically using a uracil-containing oligonucleotide template, repair activity was higher in ES cells compared with MEFs. The data are consistent with the suggestion that ES cells have multiple mechanisms, including highly active MMR and BER that preserve genetic integrity and minimize the accumulation of mutations.
Authors:
Elisia D Tichy; Li Liang; Li Deng; Jay Tischfield; Sandy Schwemberger; George Babcock; Peter J Stambrook
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-02-18
Journal Detail:
Title:  DNA repair     Volume:  10     ISSN:  1568-7856     ISO Abbreviation:  DNA Repair (Amst.)     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-22     Completed Date:  2011-07-01     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  101139138     Medline TA:  DNA Repair (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  445-51     Citation Subset:  IM    
Copyright Information:
Published by Elsevier B.V.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
DNA Repair / physiology*
DNA-Binding Proteins / metabolism
Embryonic Stem Cells / metabolism*
Fibroblasts / metabolism
Mice
Mice, Inbred C3H
Grant Support
ID/Acronym/Agency:
P30ES05022/ES/NIEHS NIH HHS; R01 ES011633/ES/NIEHS NIH HHS; R01 ES011633-05/ES/NIEHS NIH HHS; R01 ES012695/ES/NIEHS NIH HHS; R01 ES012695/ES/NIEHS NIH HHS; R01 ES012695-04/ES/NIEHS NIH HHS; R01 ES012695-04S2/ES/NIEHS NIH HHS; R01 ES12695-4S1/ES/NIEHS NIH HHS; R01ES011633/ES/NIEHS NIH HHS; T32 ES007250-21/ES/NIEHS NIH HHS; T32 ES007250-21A1/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins
Comments/Corrections

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