Document Detail


Misexpression of a PISTILLATA-like MADS-box gene prevents fruit development in grapevine.
MedLine Citation:
PMID:  23181568     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The FLESHLESS BERRY (FLB) somatic variant identified in the grapevine cultivar Ugni Blanc develops grape berries without flesh suggesting a role for the altered gene in the differentiation of flesh cells. Here we report the identification of the molecular defect responsible for this phenotype. Using a combination of genetic and transcriptomic approaches we detected the presence of a miniature inverted-repeat transposable element insertion in the promoter region of the PISTILLATA-like (VvPI) gene the grapevine homolog of Arabidopsis PISTILLATA. The transposon insertion causes specific ectopic expression of the corresponding VvPI allele during early fruit development causing the expression of genes specific for petal and stamen development within the fruit. Causal relationship between the insertion and the phenotype was demonstrated by the phenotypic and molecular analyses of somatic revertants showing that ectopic expression and mutant phenotype were always linked to the presence of the transposon insertion. The different phenotypic effects of the flb mutation on ovary morphology, fruit set and fruit development, depending on the cell lineage affected, are presented with each phenotype offering unique insights into floral and fleshly fruit development. The results highlight the importance of VvPI repression after fertilization to achieve normal fleshy fruit development and the complex genetic, genomic and cellular interactions for the flower to fruit transition in grapevine. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Authors:
Lucie Fernandez; Jamila Chaïb; José-Miguel Martinez-Zapater; Mark R Thomas; Laurent Torregrosa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-26
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Affiliation:
Instituto de Ciencias de la Vid y del Vino (CSIC, UR, Gobierno de La Rioja), C/ Madre de dios 51, 26006, Logroño, Spain; Montpellier SupAgro, UMR Amélioration et Génétique de l'Adaptation des Plantes, 2 place Pierre Viala, 34060, Montpellier Cedex, France.
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