Document Detail

Misexpression of IGF-I in the mouse lens expands the transitional zone and perturbs lens polarization.
MedLine Citation:
PMID:  11231069     Owner:  NLM     Status:  MEDLINE    
Insulin-like growth factor-I (IGF-I) has been implicated as a regulator of lens development. Experiments performed in the chick have indicated that IGF-I can stimulate lens fiber cell differentiation and may be involved in controlling lens polarization. To assess IGF-I activity on mammalian lens cells in vivo, we generated transgenic mice in which this factor was overexpressed from the alphaA-crystallin promoter. Interestingly, we observed no premature differentiation of lens epithelial cells. The pattern of lens polarization was perturbed, with an apparent expansion of the epithelial compartment towards the posterior lens pole. The distribution of immunoreactivity for MIP26 and p57(KIP2) and a modified pattern of proliferation suggested that this morphological change was best described as an expansion of the germinative and transitional zones. The expression of IGF-I signaling components in the normal transitional zone and expansion of the transitional zone in the transgenic lens both suggest that endogenous IGF-I may provide a spatial cue that helps to control the normal location of this domain.
S Shirke; S C Faber; E Hallem; H P Makarenkova; M L Robinson; P A Overbeek; R A Lang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mechanisms of development     Volume:  101     ISSN:  0925-4773     ISO Abbreviation:  Mech. Dev.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-20     Completed Date:  2001-07-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9101218     Medline TA:  Mech Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  167-74     Citation Subset:  IM    
Cell Biology and Pathology Departments, Skirball Institute for Biomolecular Medicine, Developmental Genetics Program, New York University Medical Center, 540 First Avenue, New York, NY 10016, USA.
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MeSH Terms
Cataract / genetics
Cell Differentiation
Cell Division
In Situ Hybridization
Insulin-Like Growth Factor I / biosynthesis*
Lens, Crystalline / embryology*,  metabolism*
Mice, Transgenic
Microscopy, Fluorescence
Models, Genetic
Promoter Regions, Genetic
Protein Structure, Tertiary
RNA, Messenger / metabolism
Signal Transduction
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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