| Misexpression of IGF-I in the mouse lens expands the transitional zone and perturbs lens polarization. | |
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MedLine Citation:
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PMID: 11231069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Insulin-like growth factor-I (IGF-I) has been implicated as a regulator of lens development. Experiments performed in the chick have indicated that IGF-I can stimulate lens fiber cell differentiation and may be involved in controlling lens polarization. To assess IGF-I activity on mammalian lens cells in vivo, we generated transgenic mice in which this factor was overexpressed from the alphaA-crystallin promoter. Interestingly, we observed no premature differentiation of lens epithelial cells. The pattern of lens polarization was perturbed, with an apparent expansion of the epithelial compartment towards the posterior lens pole. The distribution of immunoreactivity for MIP26 and p57(KIP2) and a modified pattern of proliferation suggested that this morphological change was best described as an expansion of the germinative and transitional zones. The expression of IGF-I signaling components in the normal transitional zone and expansion of the transitional zone in the transgenic lens both suggest that endogenous IGF-I may provide a spatial cue that helps to control the normal location of this domain. |
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Authors:
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S Shirke; S C Faber; E Hallem; H P Makarenkova; M L Robinson; P A Overbeek; R A Lang |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Mechanisms of development Volume: 101 ISSN: 0925-4773 ISO Abbreviation: Mech. Dev. Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-03-20 Completed Date: 2001-07-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9101218 Medline TA: Mech Dev Country: Ireland |
Other Details:
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Languages: eng Pagination: 167-74 Citation Subset: IM |
Affiliation:
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Cell Biology and Pathology Departments, Skirball Institute for Biomolecular Medicine, Developmental Genetics Program, New York University Medical Center, 540 First Avenue, New York, NY 10016, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cataract / genetics Cell Differentiation Cell Division Immunohistochemistry In Situ Hybridization Insulin-Like Growth Factor I / biosynthesis* Lens, Crystalline / embryology*, metabolism* Mice Mice, Transgenic Microscopy, Fluorescence Models, Genetic Phenotype Promoter Regions, Genetic Protein Structure, Tertiary RNA, Messenger / metabolism Signal Transduction Transgenes |
| Grant Support | |
ID/Acronym/Agency:
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EY10448/EY/NEI NIH HHS; EY10559/EY/NEI NIH HHS; EY10803/EY/NEI NIH HHS; EY11234/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 67763-96-6/Insulin-Like Growth Factor I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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