| Mirasol Pathogen Reduction Technology treatment does not affect acute lung injury in a two-event in vivo model caused by stored blood components. | |
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MedLine Citation:
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PMID: 19951305 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Mirasol Pathogen Reduction Technology (PRT) treatment uses riboflavin and UV light to inactivate pathogens in blood components. Neutrophil [polymorphonuclear cells (PMN)] priming activity accumulates during routine storage of cellular blood components, and this activity has been implicated in transfusion-related acute lung injury (TRALI). We hypothesize that PRT-treatment of blood components affects the priming activity generated during storage of packed RBCs (PRBCs) or platelet concentrates (PCs), which can elicit ALI in vivo. METHODS: Plasma, PRBCs and PCs were isolated from healthy donor's whole blood or by apheresis. Half of a collected unit was treated with PRT treatment and the remainder was left as an unmodified control. Supernatant was collected during storage of PCs and PRBCs and assayed for PMN priming activity and used as the second event in a two-event in vivo model of TRALI. RESULTS: PRT treatment did not induce priming activity in plasma or affect the priming activity generated during storage of PCs or PRBCs as compared with the unmodified controls. The supernatants from stored, but not fresh, PCs and PRBCs did cause ALI as the second event in a two-event animal model of TRALI, which was unaffected by PRT treatment. We conclude that the PRT treatment does not induce priming activity in plasma nor does it affect the priming activity generated during storage of PCs or PRBCs or their ability to cause ALI as the second event in a two-event in vivo model of TRALI. Moreover, the amount of priming activity in TRIMA-isolated PCs was significantly less than SPECTRA-isolated PCs. |
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Authors:
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C C Silliman; S Y Khan; J Bradley Ball; M R Kelher; S Marschner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-25 |
Journal Detail:
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Title: Vox sanguinis Volume: 98 ISSN: 1423-0410 ISO Abbreviation: Vox Sang. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-06-10 Completed Date: 2010-10-13 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0413606 Medline TA: Vox Sang Country: England |
Other Details:
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Languages: eng Pagination: 525-30 Citation Subset: IM |
Affiliation:
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The Bonfils Blood Center, Denver, Colorado, USA. christopher.silliman@ucdenver.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acute Lung Injury
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blood,
etiology* Animals Bacterial Infections / prevention & control, transmission Blood Platelets / drug effects, microbiology*, radiation effects Humans Male Platelet Transfusion / adverse effects, methods Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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P50 GM049222-15/GM/NIGMS NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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