Document Detail


Minireview: Transaminases for the synthesis of enantiopure beta-amino acids.
MedLine Citation:
PMID:  22293122     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ABSTRACT: Optically pure beta-amino acids constitute interesting building blocks for peptidomimetics and a great variety of pharmaceutically important compounds. Their efficient synthesis still poses a major challenge. Transaminases (also known as aminotransferases) possess a great potential for the synthesis of optically pure beta-amino acids. These pyridoxal 5'-dependent enzymes catalyze the transfer of an amino group from a donor substrate to an acceptor, thus enabling the synthesis of a wide variety of chiral amines and amino acids. Transaminases can be applied either for the kinetic resolution of racemic compounds or the asymmetric synthesis starting from a prochiral substrate. This review gives an overview over microbial transaminases with activity towards beta-amino acids and their substrate spectra. It also outlines current strategies for the screening of new biocatalysts. Particular emphasis is placed on activity assays which are applicable to high-throughput screening.
Authors:
Jens Rudat; Birgit R Brucher; Christoph Syldatk
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-31
Journal Detail:
Title:  AMB Express     Volume:  2     ISSN:  2191-0855     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-2-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101561785     Medline TA:  AMB Express     Country:  -    
Other Details:
Languages:  ENG     Pagination:  11     Citation Subset:  -    
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