| Minimizing creatine kinase variability in rats for neuromuscular research purposes. | |
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MedLine Citation:
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PMID: 18348763 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat serum or plasma creatine kinase (CK) activity is widely used to evaluate myopathic processes, to test the myotoxicity of different drugs, or to analyse the benefits of emerging gene therapies in some neuromuscular disorders. However, great variability is found in this determination. The aim of this study has been to control some factors of variation in order to reduce variability and increase the reproducibility of analytical data. 8-10-week-old Wistar-Han rats were used. The study consisted of four sequential phases. Phase I aimed to analyse the effect of ether and isoflurane as anaesthetic drugs. The objective of Phase II was to evaluate bleeding rats via retro-orbital sinus vs. tail vein. Phases III and IV were designed as two separate, repeated measure experiments on two factors: habituation to laboratory handling procedures in Phase III and gender in Phase IV. The repeated factor was the storage temperature of blood sample prior to centrifugation. Ether did not significantly increased the CK value. Using isoflurane, getting rats accustomed to laboratory handling procedures and whole blood refrigeration prior to centrifugation and serum separation resulted in statistically significant reduction in CK value and variability. Male rats showed significantly higher values than female rats. In the light of our findings, CK value and variability in rats may be minimized by choosing tail vein as site of bleeding, getting rats accustomed to laboratory handling procedures and maintaining whole blood refrigerated until centrifugation and serum separation. |
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Authors:
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M Goicoechea; F Cía; C San José; A Asensio; J I Emparanza; A G Gil; A López de Cerain; P Aldazabal; M Azpitarte; D Otaegui; A López de Munain |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Laboratory animals Volume: 42 ISSN: 0023-6772 ISO Abbreviation: Lab. Anim. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-03-19 Completed Date: 2008-09-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0112725 Medline TA: Lab Anim Country: England |
Other Details:
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Languages: eng Pagination: 19-25 Citation Subset: IM |
Affiliation:
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Unidad Experimental, Laboratorio Unificado, Hospital Donostia, San Sebastián, Spain. mgoicoechea@chdo.osakidetza.net |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Specimen Collection / adverse effects Creatine Kinase / blood, metabolism* Female Male Neuromuscular Diseases / blood*, metabolism Rats Rats, Inbred Strains Refrigeration Sex Characteristics Specimen Handling / adverse effects Temperature |
| Chemical | |
Reg. No./Substance:
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EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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