| Minimally invasive molecular delivery into the brain using optical modulation of vascular permeability. | |
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MedLine Citation:
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PMID: 21576460 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Systemic delivery of bioactive molecules in the CNS is hampered by the blood-brain barrier, which has bottlenecked noninvasive physiological study of the brain and the development of CNS drugs. Here we report that irradiation with an ultrashort pulsed laser to the blood vessel wall induces transient leakage of blood plasma without compromising vascular integrity. By combining this method with a systemic injection, we delivered target molecules in various tissues, including the brain cortex. This tool allows minimally invasive local delivery of chemical probes, nanoparticles, and viral vectors into the brain cortex. Furthermore, we demonstrated astrocyte-mediated vasodilation in vivo without opening the skull, using this method to load a calcium indicator in conjunction with label-free photoactivation of astrocytes. |
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Authors:
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Myunghwan Choi; Taeyun Ku; Kyuha Chong; Jonghee Yoon; Chulhee Choi |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-16 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: - ISSN: 1091-6490 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Bio and Brain Engineering, Graduate School of Medical Science and Engineering, KI for the BioCentury, and Optical BioImaging Center, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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