Document Detail


Minimal residual disease testing in acute leukemia by flow cytometry immunophenotyping: prognostic significance.
MedLine Citation:
PMID:  17353179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two main techniques are being used for the detection of minimal residual disease (MRD) in acute leukemia (AL): immunophenotypic analysis and polymerase chain reaction (PCR). In this paper, we analyze the results of assessing MRD by means of flow cytometry (FC) in a group of 93 patients with AL who were prospectively studied and treated in a single institution over a 9-year period. The presence or absence of MRD was established at a cut-off level of 2%, as judged by FC; a single result above this level was considered to define the positivity. The patients were grouped in 4 subsets: (1) acute lymphoblastic leukemia (ALL) patients with MRD (n = 36); (2) acute myeloblastic leukemia (AML) patients with MRD (n = 13); (3) ALL patients without MRD (n = 31); and (4) AML patients without MRD (n = 13). The relapse rates in these groups were 17%, 8%, 0%, and 0%, respectively, whereas the overall 7-year survival was 65%, 69%, 83%, and 98%, respectively. Our results support the usefulness of serially assessing MRD in patients with AL by means of FC; because this method is applicable to all cases of AL, despite being less sensitive than a molecular biology study; it is a good option to follow-up patients and to decide therapeutic and timely interventions.
Authors:
Guillermo J Ruiz-Argüelles; Danitza Fernández-Lara; Roberto Estrada-Gómez; Carlos Manzano; Guillermo J Ruiz-Delgado; Beatriz Pérez-Romano; Alejandro Ruiz-Argüelles
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Laboratory hematology : official publication of the International Society for Laboratory Hematology     Volume:  13     ISSN:  1080-2924     ISO Abbreviation:  Lab Hematol     Publication Date:  2007  
Date Detail:
Created Date:  2007-03-13     Completed Date:  2008-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9615688     Medline TA:  Lab Hematol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  22-6     Citation Subset:  IM    
Affiliation:
Centro de Hematología y Medicina Interna de Puebla, Puebla, Mexico. gruiz1@clinicaruiz.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Biological Markers / blood
Child
Child, Preschool
Flow Cytometry
Humans
Infant
Leukemia, Myeloid, Acute / blood*
Longitudinal Studies
Middle Aged
Neoplasm Recurrence, Local / blood*
Neoplasm, Residual / blood*,  diagnosis
Precursor Cell Lymphoblastic Leukemia-Lymphoma / blood*
Predictive Value of Tests
Survival Analysis
Chemical
Reg. No./Substance:
0/Biological Markers

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