Document Detail


Miniaturized nucleic acid amplification systems for rapid and point-of-care diagnostics: a review.
MedLine Citation:
PMID:  22704369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Point-of-care (POC) genetic diagnostics critically depends on miniaturization and integration of sample processing, nucleic acid amplification, and detection systems. Polymerase chain reaction (PCR) assays have extensively applied for the diagnosis of genetic markers of disease. Microfluidic chips for microPCR with different materials and designs have been reported. Temperature cycling systems with varying thermal masses and conductivities, thermal cycling times, flow-rates, and cross-sectional areas, have also been developed to reduce the nucleic acid amplification time. Similarly, isothermal amplification techniques (e.g., loop-mediated isothermal amplification or LAMP), which are still are emerging, have a better potential as an alternative to PCR for POC diagnostics. Isothermal amplification techniques have: (i) moderate incubation temperature leading to simplified heating and low power consumption, (ii) yield high amount of amplification products, which can be detected either visually or by simple detectors, (iii) allow direct genetic amplification from bacterial cells due to the superior tolerance to substances that typically inhibit PCR, (iv) have high specificity, and sensitivity, and (v) result in rapid detection often within 10-20 min. The aim of this review is to provide a better understanding of the advantages and limitations of microPCR and microLAMP systems for rapid and POC diagnostics.
Authors:
Farhan Ahmad; Syed A Hashsham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2012-05-03
Journal Detail:
Title:  Analytica chimica acta     Volume:  733     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-18     Completed Date:  2012-10-09     Revised Date:  2013-02-11    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-15     Citation Subset:  IM    
Copyright Information:
Published by Elsevier B.V.
Affiliation:
Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA / genetics
Equipment Design
Humans
Microfluidic Analytical Techniques / economics,  instrumentation*,  methods
Point-of-Care Systems* / economics
Polymerase Chain Reaction / economics,  instrumentation*,  methods
RNA / genetics
Chemical
Reg. No./Substance:
63231-63-0/RNA; 9007-49-2/DNA
Comments/Corrections
Erratum In:
Anal Chim Acta. 2013 Jan 14;760:100

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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