Document Detail

Miniaturized Electroosmotic Pump Capable of Generating Pressures of More than 1,200 Bars.
MedLine Citation:
PMID:  23061696     Owner:  NLM     Status:  Publisher    
The pressure output of a pump cannot be increased simply by connecting several of them in series. This barrier is eliminated with the micro pump developed in this work. The pump is actually an assembly of a number of fundamental pump units connected in series. The maximum pressure output of this pump assembly is directly proportional to the number of serially-connected pump units. Theoretically, one can always enhance the pressure output by adding more pump units in the assembly, but in reality the upper pressure is constrained by the micro tees or unions joining the pump components. With commercially available micro tees and unions, pressures of more than 1200 bars have been achieved. We have recently experimented using open capillaries to build this pump, but many capillaries have to be utilized in parallel to produce adequate flow to drive HPLC separations. In this paper, we synthesize polymer monoliths inside 75-um-i.d. capillaries, use these monoliths to assemble miniaturized pumps, characterize the performance of these pumps, and employ these pumps for HPLC separations of intact proteins. By tuning the experimental parameters for monolith preparations, we obtain both negatively- and positively-charged sub-micron capillary channels conveniently. Each monolith in a 75-um-i.d. capillary is equivalent to several thousands of open capillaries.
Congying Gu; Zhijian Jia; Zaifang Zhu; Chiyang He; Wei Wang; Aaron A Morgan; Joann J Lu; Shaorong Liu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-12
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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