| Mineralocorticoid hormone receptor and the epithelial sodium channel in a human leukemic cell line. | |
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MedLine Citation:
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PMID: 9888525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A Cell extract from the HEL (human erythroblastic leukemia) cell line was positive for both the epithelial sodium channel (ENaC) and the mineralocorticoid receptor (MCR) as glycosylated 82-84 kDa bands, and a single 102 kDa band, respectively, in Western blots using polyclonal antibodies raised against these proteins. The immunofluorescent labeling of the MCR in all cell lines showed a nucleocytoplasmic localization of the receptor whereas the ENaC was exclusively membrane-bound. These results were confirmed by confocal microscopy. The expression of the MCR in HEL cells was evident as a predicted band of 843 bp (234 amino acids) after total RNA from HEL cells had been reverse transcribed and then amplified by PCR; the ENaC was similarly evident as a predicted band of 520 bp. In both cases, near 100% identity was observed between the deduced amino acid sequences of the PCR products and those from known human sources. The multiplication of HEL cells was influenced by antagonists (RU 26752, ZK 91587) targeted for specificity to the MCR and this was reversed by the natural hormone aldosterone. These steroids also provoked chromatin condensation in the HEL population. |
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Authors:
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M Mirshahi; S Mirshahi; N Golestaneh; C Nicolas; Z Mishal; M K Agarwal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Endocrine research Volume: 24 ISSN: 0743-5800 ISO Abbreviation: Endocr. Res. Publication Date: 1998 Aug-Nov |
Date Detail:
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Created Date: 1999-04-13 Completed Date: 1999-04-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8408548 Medline TA: Endocr Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 455-9 Citation Subset: IM |
Affiliation:
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CNRS and INSERM U-86, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence
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genetics Cell Division / drug effects Chromatin / metabolism Dose-Response Relationship, Drug Epithelial Sodium Channel Humans Leukemia, Erythroblastic, Acute / metabolism*, pathology Molecular Sequence Data Osmolar Concentration RNA, Messenger / metabolism Receptors, Mineralocorticoid / antagonists & inhibitors, genetics, metabolism* Sequence Homology, Amino Acid Sodium Channels / genetics, metabolism* Spironolactone / analogs & derivatives, pharmacology Tumor Cells, Cultured / pathology |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Epithelial Sodium Channel; 0/RNA, Messenger; 0/Receptors, Mineralocorticoid; 0/Sodium Channels; 52-01-7/Spironolactone; 84542-26-7/ZK 91587 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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