| Mineralocorticoid and glucocorticoid receptor steroid binding and localization in colonic cells. | |
| | |
MedLine Citation:
|
PMID: 8166236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In rat colon epithelium glucocorticoids and mineralocorticoids regulate Na transport by binding to distinct receptors and stimulating different pathways. The distribution and intracellular localization of mineralocorticoid (MR) and glucocorticoid (GR) receptors in colonic Na-absorbing surface cells and Cl-secreting crypt cells is unknown. Surface and crypt cells were sequentially isolated from rat distal colon by EDTA chelation and mechanical dissociation. Cell viability was confirmed by trypan blue exclusion and low rates of 2',7'-bis(2-carboxyethyl)-5(6)-carboxylfluorescein leak. Histologic examination, alkaline phosphatase activity, and rates of [3H]leucine incorporation confirmed separation of surface from crypt cells. Scatchard analysis of [3H]aldosterone and [3H]triamcinolone acetonide binding demonstrated that the number of MR decreased from 7,228 +/- 1,067 in surface to 2,299 +/- 434 receptors/cell in crypt cells, whereas the number of GR increased from 20,857 +/- 4,241 in surface to 58,598 +/- 8,207 receptors/cell in crypt cells. The dissociation constants were 2.8 +/- 0.4 nM for the MR and 12 +/- 3 nM for the GR. Indirect immunofluorescence using the specific anti-MR antibody hMRsN and the anti-GR antibody BuGR-2 demonstrated that both unliganded receptors were cytoplasmic and translocated to the nucleus after hormone binding. These data indicate that both surface and crypt cells are potentially responsive to mineralocorticoids and glucocorticoids and that both the MR and GR require hormone for nuclear translocation. |
| | |
Authors:
|
G Schulman; N M Robertson; I B Elfenbein; D Eneanya; G Litwack; C P Bastl |
Related Documents
:
|
6215266 - The effect of estrogen and gestagen on the mucus production of human endocervical cells... 2992966 - Covalent cross-linking of vasoactive intestinal peptide (vip) to its receptor in intact... 7211716 - The stem-cell zone of the small intestinal epithelium. i. evidence from paneth cells in... 2230136 - An immunoassay for the quantitation of cell membrane mhc class ii antigen. 20014096 - Maintenance of pluripotency in mouse embryonic stem cells cultivated in stirred microca... 22381206 - Spatial and temporal distribution of oct-4 and acetylated h4k5 in rabbit embryos. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The American journal of physiology Volume: 266 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1994 Mar |
Date Detail:
|
Created Date: 1994-05-20 Completed Date: 1994-05-20 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: C729-40 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Temple University Health Sciences Center, Philadelphia 19140. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aldosterone
/
metabolism* Animals Colon / cytology, metabolism* Fluoresceins Fluorescent Antibody Technique Fluorescent Dyes Rats Rats, Sprague-Dawley Receptors, Glucocorticoid / metabolism* Receptors, Mineralocorticoid / metabolism* Tissue Distribution Triamcinolone Acetonide / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
R01-DK-27486/DK/NIDDK NIH HHS; S07RR05417/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fluoresceins; 0/Fluorescent Dyes; 0/Receptors, Glucocorticoid; 0/Receptors, Mineralocorticoid; 52-39-1/Aldosterone; 76-25-5/Triamcinolone Acetonide; 85138-49-4/2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Free radicals uncouple the sodium pump in pig coronary artery.
Next Document: Calcium-dependent chloride channels in endosomes from rabbit kidney cortex.