Document Detail


Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions.
MedLine Citation:
PMID:  20621767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The complex morphologies of mineralised collagen fibrils are regulated through interactions between the collagen matrix and non-collagenous extracellular proteins. In the present study, polyvinylphosphonic acid, a biomimetic analogue of matrix phosphoproteins, was synthesised and confirmed with FTIR and NMR. Biomimetic mineralisation of reconstituted collagen fibrils devoid of natural non-collagenous proteins was demonstrated with TEM using a Portland cement-containing resin composite and a phosphate-containing fluid in the presence of polyacrylic acid as sequestration, and polyvinylphosphonic acid as templating matrix protein analogues. In the presence of these dual biomimetic analogues in the mineralisation medium, intrafibrillar and extrafibrillar mineralisation via bottom-up nanoparticle assembly based on the non-classical crystallisation pathway could be identified. Conversely, only large mineral spheres with no preferred association with collagen fibrils were observed in the absence of biomimetic analogues in the medium. Mineral phases were evident within the collagen fibrils as early as 4 h after the initially-formed amorphous calcium phosphate nanoprecursors were transformed into apatite nanocrystals. Selected area electron diffraction patterns of highly mineralised collagen fibrils were nearly identical to those of natural bone, with apatite crystallites preferentially aligned along the collagen fibril axes.
Authors:
Young Kyung Kim; Li-sha Gu; Thomas E Bryan; Jong R Kim; Liang Chen; Yan Liu; James C Yoon; Lorenzo Breschi; David H Pashley; Franklin R Tay
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-02
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-10-25     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  6618-27     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ltd.
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / chemistry*
Animals
Biomimetics
Calcium / chemistry*
Cattle
Collagen / chemistry*
Hydroxides / chemistry*
Minerals / chemistry*
Organophosphonates / chemistry*
Phosphates / chemistry*
Vinyl Compounds / chemistry*
Grant Support
ID/Acronym/Agency:
R21 DE019213/DE/NIDCR NIH HHS; R21 DE019213-01/DE/NIDCR NIH HHS; R21 DE019213-02/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Hydroxides; 0/Minerals; 0/Organophosphonates; 0/Phosphates; 0/Vinyl Compounds; 1746-03-8/vinylphosphonic acid; 9003-01-4/carbopol 940; 9007-34-5/Collagen; 9159UV381P/hydroxide ion; SY7Q814VUP/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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