Document Detail


Mineral water fortified with folic acid, vitamins B6, B12, D and calcium improves folate status and decreases plasma homocysteine concentration in men and women.
MedLine Citation:
PMID:  14749760     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We investigated the effects of mineral water fortified with folic acid, vitamins B(6), B(12), D and calcium on folate concentrations in serum and erythrocytes, serum vitamin B(12) and plasma homocysteine concentrations in free-living subjects. In addition, we investigated the bioavailability of calcium added to mineral water by measuring urinary calcium excretion and serum alkaline phosphatase activity. DESIGN: Randomized, controlled, double-blinded, parallel design. SETTING: Outpatient dietary intervention with free-living subjects in Eastern Finland. SUBJECTS: Altogether, 66 subjects were recruited for the study. In all, 60 subjects completed the study. INTERVENTIONS: The study began with a 2-week run-in period followed by an 8-week intervention period. During the intervention study, subjects consumed mineral water fortified with folic acid (563 microg/day), vitamins B(6) (1 mg/day), B(12) (7.5 microg/day), cholecalciferol (0.6 microg/day) and calcium (563 mg/day) or placebo mineral water. RESULTS: The fortified mineral water increased serum and erythrocyte folate concentrations by 16.1+/-5.6 nmol/l (P<0.001) and 199+/-76 nmol/l (P<0.001), respectively, and decreased plasma homocysteine concentration by 1.6 micromol/l (P<0.001). Urinary calcium excretion and serum alkaline phosphatase activity for 24 h increased significantly (P<0.001 and P=0.01 respectively) in the intervention group. CONCLUSIONS: Mineral water fortified with folic acid, vitamins B(6), B(12) and D and calcium enhanced folate status and reduced plasma homocysteine concentration in normohomocysteinemic subjects without folate deficiency. Indirect measures of calcium and bone metabolism indicated that the calcium used in the fortification of the mineral water was bioavailable.
Authors:
N S Tapola; H M Karvonen; L K Niskanen; E S Sarkkinen
Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of clinical nutrition     Volume:  58     ISSN:  0954-3007     ISO Abbreviation:  Eur J Clin Nutr     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-29     Completed Date:  2004-04-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8804070     Medline TA:  Eur J Clin Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  376-85     Citation Subset:  IM    
Affiliation:
Contract research organization, Oy Foodfiles Ltd, Kuopio, Finland. niina.tapola@foodfiles.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Blood Pressure / drug effects
Calcium, Dietary / administration & dosage*,  blood,  urine
Female
Folic Acid / administration & dosage*,  blood
Food, Fortified*
Homocysteine / blood,  drug effects
Humans
Male
Middle Aged
Mineral Waters / administration & dosage*,  analysis
Vitamin B Complex / administration & dosage*,  blood
Vitamin D / administration & dosage*,  blood
Chemical
Reg. No./Substance:
0/Calcium, Dietary; 12001-76-2/Vitamin B Complex; 1406-16-2/Vitamin D; 454-28-4/Homocysteine; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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