Document Detail

Mineral and/or milk supplementation of fruit beverages helps in the prevention of H2O2-induced oxidative stress in Caco-2 cells.
MedLine Citation:
PMID:  21892583     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Fruit beverages are commonly supplemented with milk, vitamins and/or minerals in order to improve their healthy effects by providing some bioactive components that can act additively or synergistically against oxidative stress.
AIMS: To test whether iron, zinc, and milk added to fruit beverages do not affect the cytoprotective effect against oxidative damage to Caco-2 cells through GSH-related enzymes induction and cell cycle progression preservation, in comparison with non-supplemented fruit beverage.
METHODS: Caco-2 cells were incubated 24 h with the bioaccessible fraction (BF) of eight fruit beverages with/without iron and/or zinc, and/or milk, and then challenged with H2O2 (5 mmol L-1 -2 h). Mitochondrial enzyme activities (MTT test), GSH-Rd and GSH-Px enzyme activities, cell cycle progression and caspase-3 activity were measured.
RESULTS AND DISCUSSION: Fruit beverages prevented the deleterious effect of H2O2 on cell viability, with almost all samples reaching control basal levels. Only independent iron or zinc supplementation with/without milk exerted positive effects upon GSH-Rd activity. Both minerals with milk, afforded improved preservation of GSH-Px activity. All samples prevented the decrease in the G1 phase of cell cycle induced by H2O2, except iron supplemented samples with/without milk, but none of them avoided the increase in sub-G1 phase. However, this fact was not associated to caspase-3 activity, with a probable positive effect of zinc upon this parameter.
CONCLUSION: Mineral and/or milk supplementation of fruit beverages helps in the prevention of oxidative stress in Caco-2 cells based on cell viability maintenance, GSH-related enzymes activation, cell cycle distribution preservation and inhibition of caspase-3 activation.
A Cilla; J M Laparra; A Alegria; R Barbera
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nutrición hospitalaria     Volume:  26     ISSN:  1699-5198     ISO Abbreviation:  Nutr Hosp     Publication Date:    2011 May-Jun
Date Detail:
Created Date:  2011-09-05     Completed Date:  2012-01-06     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  9100365     Medline TA:  Nutr Hosp     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  614-21     Citation Subset:  IM    
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MeSH Terms
Caco-2 Cells
Caspase 3 / metabolism
Cell Cycle / drug effects
Cell Survival / drug effects
Dietary Supplements
Digestion / physiology
Fruit / chemistry*
Glutathione / metabolism
Glutathione Peroxidase / metabolism
Glutathione Reductase / metabolism
Hydrogen Peroxide / antagonists & inhibitors*,  pharmacology*
Minerals / pharmacology*
Mitochondria / drug effects,  enzymology
Oxidative Stress / drug effects*
Reg. No./Substance:
0/Minerals; BBX060AN9V/Hydrogen Peroxide; EC Peroxidase; EC Reductase; EC 3.4.22.-/Caspase 3; GAN16C9B8O/Glutathione

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