Document Detail


Mild reductions in mitochondrial citrate synthase activity result in a compromised nitrate assimilation and reduced leaf pigmentation but have no effect on photosynthetic performance or growth.
MedLine Citation:
PMID:  18359839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type. A more detailed physiological characterization, however, revealed that although these plants were characterized by relatively few changes in photosynthetic parameters they displayed a decreased relative flux through the tricarboxylic acid cycle and an increased rate of respiration. Furthermore, biochemical analyses revealed that the transformants exhibited considerably altered metabolism, being characterized by slight decreases in the levels of organic acids of the tricarboxylic acid cycle, photosynthetic pigments, and in a single line in protein content but increases in the levels of nitrate, several amino acids, and starch. We additionally determined the maximal catalytic activities of a wide range of enzymes of primary metabolism, performed targeted quantitative PCR analysis on all three isoforms of citrate synthase, and conducted a broader transcript profiling using the TOM1 microarray. Results from these studies confirmed that if the lines were somewhat impaired in nitrate assimilation, they were not severely affected by this, suggesting the presence of strategies by which metabolism is reprogrammed to compensate for this deficiency. The results are discussed in the context of carbon-nitrogen interaction and interorganellar coordination of metabolism.
Authors:
Agata Sienkiewicz-Porzucek; Adriano Nunes-Nesi; Ronan Sulpice; Jan Lisec; Danilo C Centeno; Petronia Carillo; Andrea Leisse; Ewa Urbanczyk-Wochniak; Alisdair R Fernie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-21
Journal Detail:
Title:  Plant physiology     Volume:  147     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-09-03     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  115-27     Citation Subset:  IM    
Affiliation:
Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476 Golm, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Carbon / metabolism*
Cell Respiration / physiology
Circadian Rhythm / physiology
Citrate (si)-Synthase / metabolism*
Citric Acid Cycle*
Cloning, Molecular
DNA, Complementary
Flowers / physiology
Fruit / physiology
Gene Expression
Isoenzymes / metabolism
Light
Lycopersicon esculentum / enzymology*,  growth & development,  physiology
Mitochondria / enzymology
Nitrates / metabolism
Nitrogen / metabolism*
Photosynthesis / physiology
Pigments, Biological / metabolism
Plant Leaves / metabolism,  radiation effects
Plants, Genetically Modified / enzymology,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/DNA, Complementary; 0/Isoenzymes; 0/Nitrates; 0/Pigments, Biological; 7440-44-0/Carbon; 7727-37-9/Nitrogen; EC 2.3.3.1/Citrate (si)-Synthase
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