| Mild preseptic hypothermia is detrimental in rats. | |
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MedLine Citation:
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PMID: 15343019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: We evaluated the effects of mild hypothermia (32 degrees C), established before experimental intra-abdominal sepsis, on outcome, cytokine pattern, and muscle tissue oxygenation. DESIGN: Clinic modeling randomized laboratory trial. SETTING: University laboratory. SUBJECTS: Ninety-six male rats. INTERVENTIONS: In a group-sequential design, using 42 rats per group, we compared mild hypothermia with normothermia before peritonitis. Peritoneal inoculation with human stool bacteria was performed to simulate clinical trial conditions. Additionally, 12 rats underwent preoperative mild hypothermia without infection. MEASUREMENTS AND MAIN RESULTS: Primary end point was mortality at 120 hrs. Secondary end points were systemic cytokine concentrations, granulocyte counts, and muscle oxygen partial pressure. Survival rate was 40% (16 of 42) after preseptic hypothermia and 62% (26 of 42) after preseptic normothermia (p =.048). All hypothermic rats without infection survived. Interleukin-10 concentrations were 1843 +/- 96 pg/mL after preseptic hypothermia, 945 +/- 225 pg/mL with preseptic normothermia, and 520 +/- 121 pg/mL after hypothermia without infection (p<.001). Macrophage inflammatory protein-2 was comparable in the treatment groups. Interleukin-6 concentrations were 106 +/- 24 pg/mL after preseptic hypothermia and 276 +/- 76 pg/mL with preseptic normothermia (p<.05). Postinfection granulocyte count was 1.7 x 10(9)/L after hypothermia and 2.4 x 10(9)/L after normothermia (p =.2). After infection, muscle oxygen partial pressure was 47 +/- 10 mm Hg with preseptic hypothermia, 85 +/- 12 mm Hg in preseptic normothermia, and 49 +/- 9 mm Hg after hypothermia without infection (p =.7). CONCLUSIONS: In this rat model of intra-abdominal sepsis, mild preseptic hypothermia (32 degrees C) reduced survival, impaired granulocyte recruitment, and changed cytokine balance, suggesting immunosuppression. |
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Authors:
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Alexander Torossian; Sebastian Ruehlmann; Martin Middeke; Daniel I Sessler; Wilfried Lorenz; Hinnerk F Wulf; Artur Bauhofer |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Critical care medicine Volume: 32 ISSN: 0090-3493 ISO Abbreviation: Crit. Care Med. Publication Date: 2004 Sep |
Date Detail:
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Created Date: 2004-09-02 Completed Date: 2004-10-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0355501 Medline TA: Crit Care Med Country: United States |
Other Details:
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Languages: eng Pagination: 1899-903 Citation Subset: AIM; IM |
Affiliation:
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Clinic of Anesthesia and Critical Care, University Hospital Marburg, Germany. torossia@med.uni-marburg.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cytokines / blood Hypothermia, Induced / adverse effects* Immunosuppression* Male Neutrophil Activation Oxygen / metabolism Peritonitis / immunology* Random Allocation Rats Sepsis / immunology, physiopathology*, prevention & control* Statistics, Nonparametric Survival Analysis |
| Grant Support | |
ID/Acronym/Agency:
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GM 061655/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 7782-44-7/Oxygen |
| Comments/Corrections | |
Comment In:
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Crit Care Med. 2004 Sep;32(9):1974-5
[PMID:
15343036
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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