Document Detail

Mild hypothermia protects rat hippocampal CA1 neurons from irreversible membrane dysfunction induced by experimental ischemia.
MedLine Citation:
PMID:  9572574     Owner:  NLM     Status:  MEDLINE    
In order to examine the effects of hypothermia on the changes in membrane potential induced by experimental ischemia (deprivation of oxygen and glucose), intracellular recordings were made from single CA1 pyramidal neurons in slice preparations of rat hippocampus. Application of ischemic medium caused irreversible changes in membrane potential consisting of an initial hyperpolarization, then a slow depolarization and a rapid depolarization. At temperatures of 35 degrees C and 37 degrees C, once the rapid depolarization occurred, readministration of oxygen and glucose failed to restore the membrane potential, a state referred to as irreversible membrane dysfunction. When the temperature was lowered to between 27 degrees C and 33 degrees C, the membrane potential returned to the control resting membrane potential in 75% of the neurons. The temperature coefficients (Q10) of the latency, the amplitude, and the maximal slope of the rapid depolarization were 2.5, 1.4 and 2.9, respectively. It is concluded that the critical neuroprotective temperature in ischemia-induced membrane dysfunction is found to be 33 degrees C in single CA1 neurons in vitro.
M Onitsuka; S Mihara; H Inokuchi; M Shigemori; H Higashi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience research     Volume:  30     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-06-16     Completed Date:  1998-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
Department of Physiology, Kurume University School of Medicine, Kurume-shi, Japan.
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MeSH Terms
Cell Membrane / drug effects,  physiology*
Glucose / pharmacology
Hippocampus / physiology,  physiopathology*
Hypothermia, Induced*
Ischemic Attack, Transient / physiopathology*,  prevention & control*
Membrane Potentials
Pyramidal Cells / drug effects,  physiology*
Rats, Wistar
Reaction Time
Reg. No./Substance:

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